新生儿先天性症状性巨细胞病毒感染与母源性原发及复发CMV感染的相关性  

Correlation with neonatal congenital cytomegalovirus infection,maternal primary infection and secondary infection

作　　者：王菊英[1] 姚琳芳[1] 李锋[1] 

机构地区：[1]宁夏医科大学总医院医学实验中心,银川750004

出　　处：《中国免疫学杂志》2017年第8期1205-1208,共4页Chinese Journal of Immunology

基　　金：宁夏自然科学基金项目(NZ14148)

摘　　要：目的:探讨新生儿先天性症状性巨细胞病毒(Cytomegalovirus,CMV)感染与母源性原发及复发CMV感染的相关性。方法:选取48例先天性症状性CMV感染新生儿及其母亲为感染组,30例未感染CMV新生儿及其母亲为阴性组,应用化学发光法(CLIA)检测两组病例外周血特异性抗体IgM/IgG(CMV-IgM/IgG)及CMV-IgG亲合力水平,荧光定量PCR法检测两组母亲乳汁、新生儿外周血及尿液中CMV-DNA含量,分析比较其检测结果的差异并回顾性分析比较了两组母亲孕早期CMV-IgG的浓度水平与本次结果的差异。结果:感染组母亲外周血CMV-IgG抗体水平及乳汁CMV-DNA阳性率显著高于对照组,差异有统计学意义(P均<0.01);病例组患儿与母亲CMV特异性IgG抗体比值小于对照组,差异有统计学意义(P均<0.01);感染组子母间IgG测定值为负相关性,差异有统计学意义(P<0.01);阴性组子母间IgG测定值为正相关性,差异有统计学意义(P<0.01);感染组母亲孕早期CMV-IgG浓度水平显著低于本次结果,差异有统计学意义(P<0.01);阴性组母亲孕早期CMV-IgG浓度水平与本次结果无显著性差异(P>0.05)。结论:孕妇体内CMV活化或再感染导致CMV-IgG水平升高,是新生儿先天性症状性CMV感染的高危因素,孕期应关注CMV-IgM/IgG动态监测。Objective ：To explore the correlation with neonatal congenital cytomegalovirus infection,matemal primar^^ infection and secondar^^ infection. Methods ： 48 neonates with congenital CMV infection were assigned to infection group with their mothers. And the other 30 couples without congenital CMV infection were assigned to negative group with their mothers. The level of CMV- IgM/IgG and affinity of CMV-IgG in peripheral blood were tested by CLIA,and CMV-DNA in mother^s milk,peripheral blood and urine of the newborn was tested by fluorescent quantitation PCR. We also analyzed the differences of the test results between the two groups and per-formed a retrospective analysis to compare the levels of CMV-IgG of the mother with early pregnancy with the result of this test. Results： In the infection group,the level of CMV-IgG in peripheral blood and CMV-DNA in milk was significantly higher than those in the negative group,the difference was statistically significant（ P〈0.01）. Ratio of CMV-IgG antibody in newborn babies and their mothers in the infection group was lower than the other group, the difference was statistically significant（ P〈0.01）. There was negative correlation of IgG level between the newborn babies and their mothers in the infection group, the difference was statistically sig-nificant （P〈0. 01） ;While in the positive correlation in the negative group, the difference was statistically significant（ P〈0. 01）. The CMV-IgG concentrations of the mothers with early pregnancy was significantly lower than that of this infection group, the difference was statistically significant（ P〈0. 01 ） ; but in the negative group, there was no significant difference between the CMV-IgG level of the mothers with early pregnancy and the results of this test（ P〉0. 05）. Conclusion ： It is a high-risk factor f

关 键 词：先天性症状性CMV感染 特异性抗体 原发感染 复发感染 既往感染 

分 类 号：R446[医药卫生—诊断学]