ADSCs干预治疗创伤性股骨颈骨折的可行性探讨  

Feasibility study of adipose-derived mesenchymal stem cells in repairing traumatic femoral neck fracture

作　　者：秦朝[1] 司艳莉[1] 官立萍[1] 郭伟[1,2] 

机构地区：[1]新乡医学院基础医学院机能实验室,新乡453003 [2]新乡医学院基础医学院化学教研室,新乡453003

出　　处：《中国免疫学杂志》2017年第8期1228-1231,1235,共5页Chinese Journal of Immunology

基　　金：2016年河南省高等学校重点科研项目(16A610012);2017年河南省科技攻关计划项目(172102210449)资助

摘　　要：目的:探讨脂肪间充质干细胞对临床上治疗创伤性股骨颈骨折的可行性。方法:采用脂肪间充质干细胞干预后进行HE染色观察创伤性股骨颈骨折大鼠骨折修复情况;免疫组化SP法检测创伤性股骨颈骨折大鼠骨折段组织血管内皮因子VEGF的表达情况;RT-q PCR检测创伤性股骨颈骨折大鼠骨折段组织血管内皮因子VEGF mRNA的表达情况。结果:ADSCs组相较于假手术组大鼠的骨愈合修复还有一定差距,但效果优于空白模型组。结论:脂肪间充质干细胞对骨组织细胞成骨修复起促进作用,利于创伤性股骨颈骨折大鼠的骨愈合。Objective ： To explore the feasibility of ADSCs in the clinical treatment of traumatic femoral neck fracture. Methods ： After the intervention by ADSCs,femoral neck fracture repair was observed by hematoxylin-eosin staining. The vascular endothelial grow-th factor expression levels at the fracture segment were quantitatively measured by immunohistochemical staining, RT-qPCR w,as utilized to detect the mRNA formation in callus tissue. Results： In the study group,we observed less fracture repair than in the sham surgery group but more than in the blank group . Conclusion： ADSCs administration can promote osseous tissue and osteoblast repair,thereby contributing to the healing of traumatic femoral neck fracture in rats.

关 键 词：ADSCS 创伤性股骨颈骨折 VEGF 

分 类 号：R373.2[医药卫生—病原生物学]