大鼠原代肝星状细胞活化过程中CCN家族表达研究  

Expression of CCN Family during Rat Primary Hepatic Stellate Cells Activation

作　　者：张艳军[1] 董育红[1] 李遂焰[1] 陈渝萍[2] 

机构地区：[1]西南交通大学生命科学与工程学院 [2]西南交通大学材料科学与工程学院,成都610031

出　　处：《天然产物研究与开发》2017年第8期1284-1289,共6页Natural Product Research and Development

基　　金：国家自然科学基金(81371675)

摘　　要：肝纤维化是肝脏损伤后的病理性修复反应,其特征是肝脏中肝星状细胞(HSC)激活、扩增和细胞外基质蛋白过度沉积。CCN蛋白因子是具信号传递功能的细胞基质蛋白,参与组织修复反应并涉及肝纤维化发生发展。本文制备了高纯度的大鼠原代HSC并通过体外培养进行激活,同步定量分析了6个CCN因子在激活过程中的表达,以期对CCN因子在HSC激活过程中的作用进行更全面的评价。Liver fibrosis is the pathological repair response of liver to injury and characteristic of activation and expanding of hepatic stellate cells( HSC) and excessive accumulation of extracelluar matrix proteins( ECM) in liver. CCN factors are the signaling matricellular protein,involving in tissue repair and fibrogenesis. In this study,the highly pure primary rat HSC was isolated and cultured for their transition into the myofibroblastic phenotype. These cultured HSCs were daily collected and characterized for the MF phenotypic features,and then mRNA expression of all CCNs genes within these transiting HSCs were analyzed with the real time QRT-PCR for a full assessment of the roles of CCNs during HSC trans-differentiation.

关 键 词：CCN家族 肝星状细胞HSC 肝纤维化 

分 类 号：Q786[生物学—分子生物学]