正交实验法优化碱水解栀子提取物制取京尼平苷酸的工艺  

Preparation of Geniposidic Acid by Alkaline Hydrolysis of Gardenia Extract

作　　者：赵帅[1] 刘磊磊[1,2] 王欢[1] 郭婕[1,2] 彭密军[1,2] 

机构地区：[1]吉首大学林产化工加工工程湖南省重点实验室 [2]湖南省植物功能成分利用协同创新中心,张家界427000

出　　处：《天然产物研究与开发》2017年第8期1349-1354,共6页Natural Product Research and Development

基　　金：湖南省自然科学基金(14JJ6037);湖南省教育厅一般项目(15C1116);吉首大学人才引进专项(jsdxrcyjkyxm201301)

摘　　要：以栀子提取物为原料,利用正交实验设计方法优化其碱水解制取京尼平苷酸的工艺。以京尼平苷酸产率为评价指标,考察了水解温度、NaOH加入量、料液比和水解时间4个单因素,采用四因素三水平正交实验设计优选得出最佳水解工艺:水解温度为70℃、NaOH加入量为14.0 m L、料液比1∶30 g/m L、水解时间为10 min,京尼平苷酸产率为11.53%。水解后溶液经过一次活性炭静态吸附和解吸,京尼平苷酸的含量可达45.33%。The conditions for the preparation of geniposidic acid from Gardenia extract by alkaline hydrolysis were optimized in this study. The effects of hydrolysis temperature,adding amount of Na OH,solid to liquid ratio,hydrolysis time were investigated and orthogonal experiments of L9( 34) were carried out. The optimal conditions were obtained as follow: hydrolysis temperature was 70 ℃,adding amount of NaOH was 14. 0 m L,solid to liquid ratio was 1∶ 30 g/m L,hydrolysis time was 10 min. Under these conditions,the yield of geniposidic acid was 11. 53%. The purity of geniposidic acid was increased to 45. 33% after static adsorption by activated carbon.

关 键 词：栀子提取物 京尼平苷 京尼平苷酸 碱水解 活性炭 

分 类 号：R284.3[医药卫生—中药学]