电导法测定低温保护剂浓度及其在低温保存中应用  

Quantitation of cryoprotective agent by electrical conductivity measurement and its application in cryopreservation

作　　者：虞效益[1,2] 陈光明[1,2,3] 胡长兴[1] 徐美娟[1] 

机构地区：[1]浙江大学宁波理工学院,浙江宁波315100 [2]浙江省制冷与低温技术重点实验室,浙江杭州310027 [3]浙江大学制冷与低温研究所,浙江杭州310027

出　　处：《浙江大学学报（工学版）》2017年第8期1640-1645,1661,共7页Journal of Zhejiang University：Engineering Science

基　　金：国家自然科学基金资助项目(51406180);宁波市自然科学基金资助项目(2016A610214);浙江省制冷与低温技术重点实验室开放基金资助项目(2016007AA)

摘　　要：为了解决低温保存生物样品时加载和去除过程中低温保护剂浓度的监测问题,提出采用电导法测定低温保护剂的浓度.测量不同温度(5~35℃)和低温保护剂质量分数(0~50%)下,二甲亚砜/氯化钠/水、甘油/氯化钠/水、乙二醇/氯化钠/水和丙二醇/氯化钠/水溶液(其中氯化钠质量分数为定值0.9%)的电导率,拟合得到电导率与低温保护剂质量分数和温度之间的关系式;通过实验证明电导法实时监测低温保护剂质量分数的可行性.结果表明,4种低温保护剂溶液的电导率均随低温保护剂质量分数的升高而减小,且符合二次多项式关系;随温度的升高而增大,且符合Vogel-Fulcher-Tammann(VFT)公式.A method of cryoprotective agent（CPA）quantitation through electrical conductivity（EC）measurement was proposed in order to monitor the addition and removal of CPA during cryopreservation of biological samples.The EC of several ternary solutions,viz.dimethyl sulfoxide/NaCl/H2 O,glycerol/NaCl/H_2O,ethylene glycol/NaCl/H_2O and propylene glycol/NaCl/H_2O,were measured at various temperatures（5-35 ℃）and CPA mass fractions（0-50%）,respectively.The mass fraction of NaCl in solution is fixed at 0.9%.An equation to fit the experimental measurements was devised to characterize the relation among EC,CPA mass fraction and temperature.The feasibility of on-site monitoring of the EC of a solution was proved by experiment. Results indicate that EC increases with increasing temperature,while the CPA has depressive influence on EC.Such behaviors of EC to CPA mass fraction and to temperature comply with the quadratic polynomial and Vogel-Fulcher-tammann（VFT）relation,respectively.

关 键 词：电导率 二甲亚砜 甘油 乙二醇 丙二醇 低温保存 

分 类 号：R318[医药卫生—生物医学工程] O657[医药卫生—基础医学]