畲药山里黄根品质生物效价检测  

Biological Titer Determination of Traditional She Medicine-Gardeniae Radix

作　　者：陈伟东[1] 刘丽仙[1] 夏丽军[1] 刘帅英 

机构地区：[1]丽水市人民医院,浙江丽水323000 [2]丽水市食品药品与质量技术检验检测院

出　　处：《中国药师》2017年第8期1463-1465,共3页China Pharmacist

基　　金：浙江省丽水市科技局项目(编号:2012CXTD11-03)

摘　　要：目的:建立畲药山里黄根的抑菌效价测定方法,测定山里黄根的抑菌效价值。方法:采用抑菌试验考察山里黄根水提液对5种标准菌株(大肠埃希菌、金黄色葡萄球菌、白色念珠菌、黑曲霉、铜绿假单胞菌)的抑菌性;采用微生物比浊仪建立山里黄根生物效价评价方法,并与HPLC法测得的竹节参皂苷Ⅳa含量进行对比。结果:山里黄根对金黄色葡萄球菌、大肠埃希菌敏感度较高;所建立的比浊法回收率在90.0%~110.0%范围内,可信限率均在5%以内;山里黄根中竹节参皂苷Ⅳa含量与效价测定结果不尽相同。结论:所建立的生物效价检测方法可作为畲药山里黄根质量评价的方法之一。Objective： To study the antimicrobial effectiveness against traditional She medicine -Gardeniae Radix and determine the titer of Gardeniae Radix. Methods： The antibacterial activity of five standard strains （Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger） was investigated. A method for the biological value evaluation of Gar- den/ae Radix was established by using a turbidity method, and compared with the content of chikusetsu saponin Ⅳ a determined by HPLC. Results： The sensitivity of Gardeniae Radix to Staphylococcus aureus and Escherichia coli was high. The recovery of the turbidity method was within the range of 90% -110%, and the reliability limit rate was less than 5%. The content of chikusetsu saponin IVa in C, ardeniae Radix was not completely consistent with the result of the bioassay method. Conclusion： The bioassay method established in the experiment can be one of the methods for the quality evaluation of Gardeniae Radix.

关 键 词：山里黄根 抑菌值 生物效价 比浊法 品质评价 竹节参皂苷Ⅳa 

分 类 号：R927.11[医药卫生—药学]