短期摄食卵黄磷脂与鱼油对高脂小鼠脂质代谢的影响  

Effects of Short-term Consumption of Fish Oil-egg-phospholipid on Lipid Metabolism in Mice Fed a High-fat Diet

作　　者：史豪豪 车红霞[1] 高翔[1] 崔洁[1] 王玉明[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《现代食品科技》2017年第7期9-16,共8页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(31371757)

摘　　要：比较研究短期摄食磷脂型(Docosahexaenoic acid,DHA)、甘油三酯型DHA以及甘油三酯型DHA与卵黄磷脂复合物对高脂小鼠脂质代谢的影响。C57BL/6J小鼠按体重分为5组,低脂对照组、高脂模型组、磷脂型DHA组、甘油三酯型DHA组、甘油三酯型DHA与蛋黄磷脂的复配组,各受试物组均采用高脂饲料,喂食一周后,分别测定血清、肝脏和脑内的脂质水平以及肝脏和脑内的脂肪酸组成。短期膳食补充DHA后均可不同程度改善小鼠的脂质代谢。磷脂型DHA以及甘油三酯型DHA与蛋黄磷脂的复配同等程度显著降低了高脂摄食小鼠的血清TG浓度;而在降低TC方面,磷脂型DHA效果优于复配型;甘油三酯型DHA与卵黄磷脂的复配组肝脏TG水平显著低于高脂组,但与磷脂型DHA组无显著差异;磷脂型DHA与复配型均显著提高肝脏和脑内DHA含量。磷脂型DHA能够更有效改善高脂饮食小鼠血清和肝脏脂质代谢,且磷脂型DHA在增加脑内DHA方面效果最佳。The effects of short-term consumption of docosahexaenoic acid（DHA）-enriched phospholipids, DHA-enriched triglycerides, and the combination of DHA-enriched triglycerides and egg-phospholipids on lipid metabolism in mice fed a high-fat diet were compared. C57BL/6J mice were divided into five groups： low-fat group, high-fat group, DHA-enriched phospholipid group, DHA-enriched triglyceride group, and DHA-enriched triglyceride-egg-phospholipids combination group. Mice were fed the high-fat diet for a week; thereafter, lipid levels in the serum, liver, and brain were measured, along with the fatty acid compositions in the liver and brain. The short-term dietary supplementation of DHA could improve lipid metabolism in mice to different degrees. The serum triglyceride（TG） concentration was significantly reduced by DHA-enriched phospholipids and by the combination of DHA-enriched triglycerides and egg-phospholipids to the same degree. DHA-enriched phospholipids were superior to the combination of DHA-enriched triglycerides and egg-phospholipids in decreasing total serum cholesterol. The hepatic TG level in the combination group was notably lower than that in the high-fat group. However, no significant difference was observed in the hepatic TG level between the DHA-enriched phospholipids group and the combination group. Furthermore, DHA-enriched phospholipids and the combination of DHA-enriched triglycerides and egg-phospholipids could significantly increase the content of DHA in the liver and brain. DHA-enriched phospholipids could more effectively improve lipid metabolism in the serum and liver of mice fed a high-fat diet, and showed the highest increase in brain DHA level.

关 键 词：脂质代谢 二十二碳六烯酸 甘油三酯 磷脂 脂肪酸组成 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]