广州地区吸食毒品人员丙型肝炎病毒感染的分子流行病学研究  

Molecular epidemiology of hepatitis C virus infections among intranasal drug users in Guangzhou,China

作　　者：王敏[1] 廖峭[1] 许茹[1] 熊华平[1] 宋丹丹[2] 尤庆柱 单振刚[1] 黄杰庭[1] 黄珂[1] 戎霞[1] 付涌水[1] 

机构地区：[1]广州血液中心临床输血研究所,广东广州510095 [2]南方医科大学生物技术学院,广东广州510515

出　　处：《中国病毒病杂志》2017年第3期201-206,共6页Chinese Journal of Viral Diseases

基　　金：广东省医学科学技术研究基金项目(A2016310);广东省自然科学基金重点项目(2014A030311005);广东省科技计划项目(2016A020216001);广州市科技计划项目(201509010009)

摘　　要：目的了解广州地区男性吸食毒品人群丙型肝炎病毒(hepatitis c virus,HCV)感染率以及分子流行病学。方法收集2011年1~12月广州地区男性吸食毒品人员共418份外周静脉血标本。采用2种ELISA试剂对标本进行抗-HCV检测,双试剂呈反应性的标本进行HCV RNA检测,RNA阳性者通过HCVE1和NS5B基因片段的序列测定获得HCV基因分型;RNA阴性的标本采用重组免疫印迹试验(recombinant immunoblot assay,RIBA)对抗-HCV进行确证。采用SPSS进行统计分析,P<0.05为差异有统计学意义。结果 418份标本中,HCV RNA阳性118份,HCV RNA阴性、RIBA确证阳性14份,HCV感染率为31.58%(132/418),HCV感染者中RNA阳性率为89.39%(118/132)。HCV感染率与吸毒开始年份(χ2=36.08,P=2.79×10-7)以及吸毒时间的长短(χ2=17.62,P=0.001)有关。93例标本成功获得6种HCV基因分型:1a(6.45%)、1b(5.38%)、3a(7.53%)、3b(23.66%)、6a(55.91%)和6e(1.07%)。结论广州地区男性吸食毒品人员HCV感染率较高;2001年之后开始吸毒的人的HCV感染率显著低于2001年之前开始吸毒的人群,吸毒时间越长,HCV感染率越高;HCV 6a和3b为该人群主要的流行亚型。Objective To study the prevalence and molecular epidemiology of hepatitis c virus（HCV）infections among intranasal drug users in Guangzhou of China. Methods A total of 418 male intranasal drug users were recruited from January to December 2011 in Guangzhou,China.Anti-HCV antibody was detected by two ELISA assays.Samples reactive to both ELISA assays were subsequently tested for the presence of HCV RNA.Viral genotypes of the HCV RNA（＋）samples were determined based on the sequences of HCV E1 and NS5Bgene fragments.Samples with undetectable HCV RNA were further confirmed for the presence of anti-HCV antibody using recombinant immunoblot assay（RIBA）.SPSS software was used for statistical analysis. Results Among 418 subjects,118were positive for HCV RNA,14 were negative for HCV RNA but positive for RIBA,the rate of HCV infection was 31.58%（132/418）,and the rate of RNA positive among HCV infected subjects was 89.39%（118/132）.The rate of HCV infection was associated with the initiation time（χ^2=36.08,P=2.79×10^-7）and the duration time（χ^2=17.62,P=0.001）of drug use.Six virus genotypes/subtypes were identified among 93specimens：HCV-1a（6.45%）,-1b（5.38%）,-3a（7.53%）,-3b（23.66%）,-6a（55.91%）and-6e（1.07%）. Conclusions High rate of HCV infection was found in male intranasal drug users.HCV infection rate was significantly reduced among population who started drug abuse after 2001 than those before 2001.The rate of HCV infection increased with the longer duration of drug use.HCV 6aand 3bwere dominant subtypes among male intranasal drug users in Guangzhou,China.

关 键 词：丙型肝炎病毒 基因分型 吸食毒品人员 分子流行病学 

分 类 号：R181.3[医药卫生—流行病学] R512.63[医药卫生—公共卫生与预防医学]