人脐带间充质干细胞条件培养基对舌鳞癌Cal-27细胞增殖的影响  

Effects of human umbilical cord mesenchymal stem cell conditioned medium on proliferation of tongue squamous cell carcinoma Cal-27

作　　者：徐玥[1] 李涛[2] 董刚[2] 李凤梅[2] 于珊珊[1] 李娜娜[3] 黄磊[1] 郑建金[2] 

机构地区：[1]青岛大学附属青岛市市立医院,山东青岛266000 [2]青岛市市立医院口腔医学中心,山东青岛266000 [3]潍坊医学院,山东潍坊261000

出　　处：《临床口腔医学杂志》2017年第7期403-406,共4页Journal of Clinical Stomatology

基　　金：青岛市科技局重点项目(15-9-2-79-nsh)

摘　　要：目的:探讨人脐带间充质干细胞(HUC-MSCs)条件培养基对舌鳞癌Cal-27细胞生物学特性的影响。方法:培养HUC-MSCs和Cal-27细胞,倒置显微镜下观察细胞形态;将HUC-MSCs条件培养基与Cal-27细胞体外间接共培养,CCK-8法检测Cal-27细胞的增殖活性,绘制生长曲线;通过Western blot法检测HUC-MSCs条件培养基对Cal-27细胞凋亡蛋白及Wnt/β-catenin信号通路关键蛋白的表达。结果:生长曲线结果显示,HUC-MSCs条件培养基可抑制Cal-27细胞增殖,并呈一定的浓度依赖性,这些变化伴随Cal-27细胞内PARP-1表达上调,Bcl-2、β-catenin、cMyc表达下调。结论:HUC-MSCs条件培养基可抑制Cal-27细胞增殖并促使其凋亡,这可能与细胞内的Wnt/β-catenin信号通路被抑制有关。Objective ：To investigate the effect of human umbilical cord mesenchymal stem cells（ HUC-MSCs） on the biological characteristics of tongue squamous cell carcinoma Cal-27 cells, and to provide a new idea for the treatment of TSCC. nethods：HUC-MSCs and Cal-27 cells were cultured and observed under inverted microscope. The proliferative activity of Cal-27 cells was detected by CCK-8 method. The proliferation of Cal-27 ceils was observed by co-culture of HUC-MSCs conditioned medium with Cal-27 cells. The expression of apoptotic protein and Wut / ^-catenin signaling pathway in Cal-27 cells was detected by Western blot. Results：The results of growth curve showed that HUC-MSCs could inhibit the proliferation of Cal-27 cells in a dose-dependent manner. These changes were associated with up-regulation of PARP-1 expression in Cal-27 ceils, and Bcl-2 and β-catenin, C-Myc expression down. Conclusion：HUC-MSCs conditioned medium can inhibit the proliferation and induce apoptosis of Cal-27 cells ,which may be related to the inhibition of Wnt / β-catenin signaling path way.

关 键 词：人脐带间充质干细胞 条件培养基 舌鳞癌细胞 增殖 凋亡 WNT信号通路 

分 类 号：R813.2[医药卫生—放射医学]