临床微生物形态学及特殊耐药性检测室间质量评价体系的建立及应用  

Establishment and application of external quality assessment system for microbial morphology and detection of special drugresistance in clinical laboratory

作　　者：魏莲花[1] 邹凤梅[1] 刘刚[1] 杨永清[1] 王晚霞[1] 吴玲[1] 李军春[1] 王欣[1] 陈玉芊[1] 陈晓青[2] 李可可[2] 邵海鑫 朱吉超[3] WEI Lian-hua ZOU Feng-mei LIU Gang YANG Yong-qing WANG Wan-xia WU Ling LI Jun-chun WANG Xin CHEN Yu- qian CHEN Xiao-qing LI Ke-ke SHAO Hai-xing ZHU Ji-chao(Department of Clinical Laboratory, Gansu Provincial Center for Clinical Laboratory, Gansu Provincial Hospital, Lanzhou 730000, Gansu Gansu University of Chinese Medicine, Lanzhou 730000, Gansu School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia, China)

机构地区：[1]甘肃省人民医院检验科、甘肃省临床检验中心,兰州730000 [2]甘肃中医药大学,兰州730000 [3]宁夏医科大学临床医学院,银川750004

出　　处：《临床检验杂志》2017年第7期538-541,共4页Chinese Journal of Clinical Laboratory Science

摘　　要：目的研究临床微生物形态学及特殊耐药性检测室间质量评价(external quality assessment,EQA)体系的建立、运行与实施,探讨该体系的临床应用价值。方法每年2次定期在甘肃省对各参评实验室发放已知的临床微生物细(真)菌落、革兰染色、抗酸染色图片,以及美国临床和实验室标准化协会(Clinical and Laboratory Standards Institute,CLSI)标准更新的知识点(特殊耐药性检测)结果图片。要求各参评单位在规定时间内完成判读并上报结果。回报结果以完全符合、基本符合和不符合的形式进行总结、分析。结果临床微生物形态学及特殊耐药性检测EQA体系运行2年,共进行24次,年度完全符合率逐年上升,2015年达到91.3%。结论临床微生物形态学及CLSI标准知识点考核EQA体系可督导二级以上医院临床微生物检验人员掌握形态学识别技能及学习CLSI知识点,全面提高临床微生物人员专业技能。Objective To investigate the establishment,operation and performance of external quality assessment（ EQA） system for microbial morphology and detection of special drug-resistance in clinical laboratory,and explore the value of the developed system in clinical application. Methods The pictures of known bacteria and fungi colony,gram staining and acid-fast staining from clinical microbiology were distributed to the participating laboratories in Gansu province twice a year at regular intervals. The pictures of standard knowledge points from CLSI,such as special drug resistance were distributed simultaneously. All the participating laboratories were required to complete the interpretation for the pictures and report their results in a scheduled time. Then the results were summarized and analyzed as 3 modes： complete consistency,general consistency and non-consistency. Results During the 2 years when the EQA system for microbial morphology and detection of special drug-resistance were performed for 24 times,the rate of annual complete consistency increased year by year and reached to 91. 3% in 2015. Conclusion The EQA system based on the examinations of microbial morphology and CLSI standard knowledge points for clinical laboratory may supervise the staff of clinical microbiology laboratories in the hospitals at second grade or above to master the skills of morphological identification and learn CLSI knowledge points,so their professional skills of clinical microbiology could be comprehensively improved.

关 键 词：微生物形态学 CLSI 特殊耐药 室间质量评价 

分 类 号：R446.5[医药卫生—诊断学]