牛乳头状瘤病毒2型贵州株L2基因克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of L2 Gene of BPV2 from Guizhou Province

作　　者：张海[1] 杨丽彦[2] 张军 冯旭芳 周碧君[1] 王开功[1] 文明[1] 程振涛[1] 王伟[1] 胡兴义 ZHANG Hai YANG Li-yan ZAHGN Jun FENG Xu-fang ZHOU Bi-jun WANG Kai-gongl WEN Ming CHENG Zhen-tao WANG Wei HU Xing-yi(College of Animal Science, Guizhou University, Guiyang, 550025, China Guizhou Light Industry Vocational and Technical College Guiyang , 550025, China Animal Health Supervision Institute of Liupanshui City, Lupaushui, Guizhou, 553001, China Defiang County Animal Disease Prevention and Control Center, Dejiang, Guizhou 565200, China)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州轻工职业技术学院,贵阳550025 [3]贵州省六盘水市动物卫生监督所,贵州六盘水553001 [4]贵州省德江县动物疫病预防控制中心,贵州德江565200

出　　处：《中国兽药杂志》2017年第8期14-22,共9页Chinese Journal of Veterinary Drug

基　　金：贵州省黔南州社会发展科技重大计划项目[黔南社发科(20140325)];贵州省农业委员会科技专项(黔农财[2015]149号)

摘　　要：为分析牛乳头状瘤病毒2型贵州株(BPV2-GZ01株)L2基因的分子特征,预测编码蛋白的生物学功能,对BPV2-GZ01株L2基因进行PCR扩增、克隆及序列测定,应用生物信息学相关软件及方法,对BPV2-GZ01株L2基因进行序列分析并对其编码蛋白进行二级结构、三级结构、B细胞表位、保守结构域、跨膜结构域和信号肽预测。结果显示,L2基因PCR扩增产物大小为1404 bp,编码467个氨基酸;与参考株BPV2株、BPV2-SW01株、BPV2-AKS01株、BPV13株、BPV1株相应序列核苷酸同源性分别为99.0%、99.7%、99.3%、83.9%和75.1%,氨基酸的同源性分别为98.9%、99.6%、99.4%、89.5%和82.7%;系统进化显示,BPV2-GZ01株L2基因与BPV2-SW01株亲缘关系最近;二级结构以无规则卷曲、β-折叠和α-螺旋区域所占比例较大,预测此蛋白可能存在B细胞优势抗原表位,无跨膜区域,无信号肽区域。本研究结果将为贵州省BPV核酸疫苗研究提供理论依据。In order to study and analyze the L2 gene of Bovine papillamavirus 2 in Guizhou province,the L2 gene of BPV2-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis,transmembrane domain and signal peptide of L2 gene were predicted. The results indicated the length of L2 gene was 1404 bp,encoding 467 amino acids. The L2 gene of BPV2-GZ01 strain shared a nucleotide identities of 99. 0%、99. 7%、99.3%、83.9% and 75.1%,and an amino acid identities of 98.9%、99.6%、99.4%、89.5% and 82.7% with those of strains BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1,respectively. The results of phylogenetic tree analysis indicated that there was a close relationship between BPV2-GZ01 strain and BPV2-SW01. Prediction of the secondary structure of L2 indicated that the random coil,extended strand and alphahelix took a higher percentage. The L2 protein was supposed contain antigen epitopes,and have a transmembrane domains,no signal peptide found. Tertiary structure is curved spiral structure. These results provided a theoretical basis for further research of nucleic acid vaccine of BPV.

关 键 词：牛乳头状瘤病毒2型 贵州株 L2基因 生物信息学分析 

分 类 号：S852.65[农业科学—基础兽医学]