热带无爪螨主要变应原Blo t 21抗原表位的预测  

Prediction of epitopes of the major allergen Blo t 21 from Blomia tropicalis

作　　者：肖正泮 韦双双[2] 关怀[2] 王大勇[2] 裴业春[1] XIAO Zheng-pan WEI Shuang-shuang GUAN Huai WANG Da-yong PEI Ye-chun(College of Animal Science and Technology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228 College of Biological Science, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China)

机构地区：[1]海南大学热带农林学院动物科技学院,海南海口570228 [2]海南大学热带农林学院生物学院,海南海口570228

出　　处：《热带医学杂志》2017年第7期841-845,858,共6页Journal of Tropical Medicine

基　　金：国家自然科学基金青年项目(31402257);海南省科技项目(ZDYF2017110);海南省自然科学基金(20153084)

摘　　要：目的预测热带无爪螨主要变应原Blo t 21的抗原表位。方法利用DNAStar Protean软件分析热带无爪螨主要变应原Blo t 21的二级结构和表面特性,如理化性质、亲水性、表面可及性、柔韧性等,预测Blo t 21蛋白的B抗原表位和T抗原表位,同时利用Bepi Pred在线分析预测其可能的B细胞抗原表位。结果 Blo t 21蛋白的二级结构以α螺旋为主,存在7个潜在的B抗原表位位点,可能的B抗原表位区域有:2-11,19-28,35-54,55-70,72-83,96-112,123-129氨基酸残基或其附近。含有8个潜在的T细胞抗原表位区域:13-17,22-31,37-46,51-64,69-75,80-84,95-99,102-105氨基酸残基或其附近。结论应用Protean软件和Bepi Pred在线预测了热带无爪螨主要变应原Blo t 21的抗原表位,为进一步研究Blo t 21变应原的结构与功能,研制针对热带螨的预防性和治疗性疫苗、开发相应的诊断试剂盒奠定了基础。Objective To predict the epitopes of the major allergen Blo t 21 from Blomia tropicalis. Methods The secondary structure and surface properties of Blo t 21 allergen were analyzed by DNAStar Protean software ,such as physical and chemical characters,hydrophilicity,surface probability and flexibility. Then the B cell epitopes and T cell epitopes of Blo t 21 were predicted by DNAStar software. At the meanwhile ,the Bcell epitopes for Blo t 21 were predicted with the online software BepiPred. Results The secondary structure of Blo t 21 allergen was rich in alpha helix. It contained seven potential B cell epitopes located at 2-11,19-28,35-54,55-70,72-83,96-112,123-129 amino acid residues or nearby. There were eight potential T cell epitopes located at 13-17,22-31,37-46,51-64,69-75,80-84,95-99,102-105 amino acid residues or nearby. Conclusion The epitopes of the major allergen Blo t 21 from Blomia tropicalis were predicted by DNAStar Protean and BepiPred software. The results could provide a base for the studies of structure and function of Blo t 21 allergen,development of the preventive and therapeutic vaccines for Blomia tropicalis,and diagnostic kit for the detection of allergies induced by Blomia tropicalis.

关 键 词：热带无爪螨 抗原表位 变应原 BLO t 21 

分 类 号：R384.4[医药卫生—医学寄生虫学]