siRNA干扰内源性TM9SF1基因对人脐静脉内皮细胞炎症因子及血管紧张素转化酶1表达的影响  

Effects of interference of siRNA with endogenous TM9SF1 on the expressions of inflammation-related genes and ACE1 in human umbilical vein endothelial cells

作　　者：张国英[1] 杨朵[1] 高娜娜[1] 唐子华[1] 李娜[1] 仝永娟[1] 肖娟[2] Zhang Guoying Yang Duo Gao Nana Tang Zihua Li Na Tong Yongjuan Xiao Juan(Department of Central Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China Department of Neurology, Xiangyang Central Hospital, Hubei University of Arts and Science, Xiangyang 441021, China)

机构地区：[1]首都医科大学附属北京世纪坛医院中心实验室,北京100038 [2]湖北文理学院附属襄阳市中心医院神经内科,襄阳441021

出　　处：《广西医科大学学报》2017年第8期1127-1130,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81601373);湖北省自然科学基金资助项目(No.2016CFB407);首都医科大学附属北京世纪坛医院院国青基金资助项目(No.2016-QB04)

摘　　要：目的:探讨siRNA干扰内源性TM9SF1基因对人脐静脉内皮细胞(HUVEC)炎症因子及血管紧张素转化酶1(ACE1)表达的影响。方法:将HUVEC分为阴性对照组(si-NC组)和转染组(si-TM9SF1组)。以脂质体Lipofectamine 3000将TM9SF1基因特异性siRNA转染至HUVEC细胞,采用实时荧光定量PCR(qPCR)方法检测特异性siRNA对TM9SF1基因的干扰效果,并比较两组白介素(IL)-1β、IL-8和ACE1基因的相对表达量。结果:转染TM9SF1基因特异性siRNA 48h后,si-TM9SF1组细胞中TM9SF1基因相对表达量明显低于si-NC组(P<0.05),且si-TM9SF1组IL-1β、IL-8和ACE1基因相对表达量亦明显低于si-NC组(P<0.05或P<0.001)。结论:干扰内源性TM9SF1可明显抑制HUVEC中炎症因子IL-1β、IL-8及血管收缩相关基因ACE1的表达。Objective：To study the effects of the interference of siRNA with endogenous TM9SF1 gene on the inflammation-related genes and angiotensin converting enzyme 1 （ACE1） in human umbilical vein endo- thelial cells （HUVEC）. Methods：The experiment was divided into a negative control group （si-NC group） and a interference group （si-TM9SF1 group）. The specific siRNA of TM9SF1 was synthesized and trans- fected into HUVEC. The mRNA expressions of interleukin （IL）-1β, IL8 and ACE1 were detected by real- time fluorescence quantitative PCR （qPCR）. Results： The mRNA expression of TM9SF1 in si-TM9SF1 group was significantly lower than that in si-NC group 48 h after the transfection （P〈0.05）. The mRNA expressions of IL-1β, IL-8 and ACE1 in si-TM9SF1 group were down-regulated, compared to si-NC group （P〈0.05 or P〈0. 001）. Conclusion： Interference of siRNA with endogenous TM9SF1 could significantly inhibit the expression of inflammation related genes （IL-1β, IL-8） and ACE1 in HUVEC.

关 键 词：TM9SF1 内皮细胞 RNA干扰 炎症相关基因 ACE1 

分 类 号：R543.5[医药卫生—心血管疾病]