参贝消瘿颗粒的HPLC特征图谱  

HPLC specific chromatogram of Shenbei Xiaoying Granules

作　　者：张磊[1,2] 马海春 苏酩[2] 刘善新[2] 王杰[4] 靳光乾[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省中医药研究院,山东济南250014 [3]菏泽市食品药品检验检测研究院,山东菏泽274000 [4]山东大学附属省立医院,山东济南250021

出　　处：《山东科学》2017年第4期1-5,共5页Shandong Science

基　　金：山东省科技发展计划(2014GGH218026);济南市科技计划(201219008);济南市高校院所计划(201401255)

摘　　要：建立了参贝消瘿颗粒的HPLC特征图谱。采用ODS-2 Hypersil C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈为流动相A,以0.1%磷酸水溶液为流动相B,梯度洗脱,柱温30℃,流速1.0 m L/min,检测波长225 nm,进样量5μL。建立的特征图谱具有良好的精密度、重复性和稳定性,9批样品特征图谱的相似度均大于0.95,确定了5个共有特征峰,并指认出其中2个为芍药苷、迷迭香酸,同时通过对单味药材的特征图谱的对比分析确定各特征峰的来源。该方法准确可靠,专属性强,可用于参贝消瘿颗粒的质量控制。The HPLC specific chromatogram of Shenbei Xiaoying Granules was established. Specific chromatogram was carried out on a ODS-2 Hypersil C18column（ 250 mm × 4. 6 mm,5 μm）,with gradient elution consisting of acetonitrile as mobile phase A and 0. 1% phosphoric acid solution as mobile phase B. Column temperature was 30 ℃,flow rate was1. 0 m L·min^-1,detection wavelength was 225 nm,and sample volume was 5 μL. The established HPLC specific chromatogram of Shenbei Xiaoying granules has good precision,repeatability and stability. The similarity of characteristic maps of 9 batches of Shenbei Xiaoying Granules was all greater than 0. 95. Five common specific chromatogram peaks were determined,and two peaks were identified as paeoniflorin and rosemary acid. At the same time,the source of each characteristic peak in HPLC specific chromatogram was determined by comparing and analyzing the HPLC specific chromatogram of single medicinal herb. This method is accurate,reliable and specific,which can be used for the quality control of Shenbei Xiaoying Granules.

关 键 词：参贝消瘿颗粒 HPLC 特征图谱 

分 类 号：R284.1[医药卫生—中药学]