土壤杆菌固氮、溶磷活性及对植物的促生作用  

Nitrogen fixation,phosphate solubilizing activity and plant growth promotion of Agrobacterium

作　　者：魏艳丽[1] Maarten RYDER 李纪顺[1] 李玲[1] 杨玉忠[1] 杨合同[1,3] 

机构地区：[1]山东省科学院生态研究所、山东省应用微生物重点实验室,山东济南250014 [2]澳大利亚阿德莱德大学农业食品葡萄酒学院,阿德莱德5064 [3]山东省黄河三角洲可持续发展研究院,山东东营257000

出　　处：《山东科学》2017年第4期26-30,共5页Shandong Science

基　　金：山东省重点研发计划(2016GNC113010);2017年度山东省引进国外智力成果示范推广项目;黄河三角洲学者工程专项

摘　　要：通过选择性培养基,定性和定量检测了26株土壤杆菌的固氮和溶磷活性,通过盆栽检测了澳洲生防菌株K1026对黄瓜和小麦的促生长作用。结果显示,包括致病性和无致病性的26株土壤杆菌均可在无氮培养基上正常生长;其中19株具有溶磷活性,K1026对磷酸钙液体培养基的溶磷量为109.11μg/m L;对黄瓜幼苗根系生长具有显著促进作用,但对小麦生长没有明显影响。Through selective medium,the function of fixing nitrogen and dissolving phosphorus of 26 Agrobacterium strains were studied by both qualitative and quantitative analysis methods. Meanwhile,growth-promoting effect of Australian biocontrol strain K1026 on cucumber and wheat was measured by pot experiment. The results showed that all the strains regardless of pathogenicity or non-pathogenicity could grow normally on nitrogen-free medium. 19 of the 26 strains had the ability to dissolve phosphorus,the Phosphate released by K1026 in Pikovskaya broth medium was up to 109. 11 μg/m L,having the strongest activities among all stains. The pot experiments showed that K1026 had obvious growth-promoting effect on the cucumber seedlings,and the root length and the dry weight of the root biomass was increased by 25. 49%,62. 96%,respectively,compared with the control. However,K1026 had no obvious effect on the growth-promotion of wheat.

关 键 词：土壤杆菌 AGROBACTERIUM RHIZOGENES K1026 固氮 溶磷 促生作用 

分 类 号：S436.629[农业科学—农业昆虫与害虫防治]