蛇类中药饮片分子鉴别方法的研究进展  

Progress in molecular identification of snake drugs

作　　者：许翊[1] 陆妍[1] 武海萍[2] 卜莹[2] 

机构地区：[1]中国药科大学,江苏南京210009 [2]南京军区联勤部药品仪器检验所,江苏南京210008

出　　处：《中国中药杂志》2017年第15期2930-2933,共4页China Journal of Chinese Materia Medica

基　　金：南京军区医学科技创新重大课题(13ZX40)

摘　　要：蛇类中药饮片具有较高的临床药用价值,具有抗炎、镇痛、通络等功效,药理作用明显。近年来,由于野生动植物资源匮乏、蛇类中药饮片价格昂贵,市场上出现了很多伪品和混淆品。传统的中药饮片鉴别方法包括基源、形态、显微、理化鉴别四大方法体系,但这些方法都存在一些弊端,包括对中药样本有较高的形态学要求、鉴别过程复杂、易受主观因素影响等。随着分子生物学研究的深入和分子遗传标记技术的发展,中药鉴别方法逐渐多元化,一些新兴的理论和技术被引入中药材鉴别领域,RAPD技术、PCR反应、DNA条形码分析等。该文将着重对当前蛇类中药饮片的分子鉴别方法作简要综述。Snake drugs have high values in clinical medication for anti-inflammatory, analgesia activities and dredging collaterals. However, owing to their deficient resource and substantial profit, many counterfeits for snake drugs have appeared on the market. Tra- ditional methods for Chinese medicine authentication include identification of origin, morphology identification, microscopy and physio- chemical identification. But these methods are restricted in application because of their high morphological requirement for specimens, complex process for assays and indeterminate results guided by subjective. With the development of molecular biology and molecular genetic techniques, new theories and technologies for molecular detection have been introduced to the authentication of Chinese medi- cine, such as RAPD, specific PCR amplification, DNA barcoding analysis and so on, improved the authentication system of Chinese medicine. Here, we will give a brief review of molecular detection methods for snake drugs authentication.

关 键 词：蛇类中药饮片 RAPD PCR DNA条形码分析 

分 类 号：R282.5[医药卫生—中药学]