微型化介质研磨法制备槲皮素纳米混悬剂  

Preparation of nanosuspension of quercetin with a miniaturized milling method

作　　者：刘肖[1,2] 刘娟[1] 庞建云[1] 申宝德[2] 沈成英[2] 连王权 李小芳[1] 袁海龙[2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国人民解放军空军总医院药学部,北京100142

出　　处：《中国中药杂志》2017年第15期2984-2988,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81573697);后勤科研重点项目(BKJ16J011)

摘　　要：采用Box-Behnken设计-效应面法优化一种微型化介质研磨法制备槲皮素纳米混悬剂(QT-NS)的工艺参数并评价所制得的QT-NS的体外累积释放度。结果显示,以最优工艺参数:氧化锆珠子4.5 m L、研磨速度690 r·min-1、研磨时间1.5 h,制得的QT-NS的粒径为(169±5)nm,多分散度指数为0.204±0.006,稳定系数为0.827±0.014,且理论预测值与实测值偏差较小,模型具有良好的预测性;在120 min体外累积释放度实验中,QT-NS明显高于原料药和物理混合物。使用该种微型化介质研磨法能成功制备QT-NS,且该方法中原料药消耗少,工艺简单、操作方便,可为纳米制剂的制备提供新的参考。The nanosuspension of quercetin （QT-NS） was prepared by a miniaturized milling method, and the process was opti- mized by Box-Behnken response surface method. Then the accumulative release rate of QT-NS in vitro was determined. The resuhs showed that the optimal process parameters were as follows ： ZrO24. 5 mL, milling speed 690 r · min - 1 and milling time 1.5 h ; the par- ticle size of QT-NS was （ 169 ± 5 ） nm, polydispersity index of 0. 204 ±0. 006 and stability index of 0. 827 ± 0. 014, respectively. There was a little deviation between the theoretically predicted value and the measured value, indicating that this model had a good pre- diction effect. The accumulative release rate in vitro of QT-NS in 120 min was significantly higher than that of the raw drug and physical mixture. This simple low-cost miniaturization approach could prepare QT-NS successfully, and could provide reference for the formula- tion of the nanosuspension.

关 键 词：槲皮素 纳米混悬剂 微型化介质研磨法 体外释放 

分 类 号：R283.6[医药卫生—中药学]