ERK5和MMP-9在骨肉瘤中的表达及其临床意义  

ERK5 and MMP-9 expression levels in osteosarcoma and their clinical significance

作　　者：王建澍[1,2,3] 移志刚[1,2] 蒲彦川 宋建民[3] 耿彬[1,2] 康雅琼[3] 马淑萍[3] 安丽萍 夏亚一[1,2] 

机构地区：[1]兰州大学第二医院骨科,兰州市730030 [2]甘肃省骨关节疾病研究重点实验室 [3]甘肃省肿瘤医院骨软组织肿瘤科

出　　处：《中国肿瘤临床》2017年第14期689-694,共6页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:81672207)资助~~

摘　　要：目的:探讨细胞外信号调节激酶5(extracellular signal-regulated kinase 5,ERK5)蛋白和基质金属蛋白酶-9(matrix metallo proteinase-9,MMP-9)在骨肉瘤组织中的表达及其临床意义。方法:选取71例骨肉瘤组织和40例正常骨组织,采用免疫组织化学法检测ERK5和MMP-9的表达,并分析ERK5和MMP-9的表达与骨肉瘤患者临床病理特征及预后之间的关系。结果:骨肉瘤组织中ERK5和MMP-9阳性表达率分别为85.9%(61/71)、74.65%(53/71),均明显高于正常骨组织中阳性表达率12.5%(5/40)、10.0%(4/40),差异均具有统计学意义(均P<0.05)。ERK5和MMP-9的阳性表达与Enneking分期和转移相关(均P<0.05)。Kaplan-Meier生存分析显示在骨肉瘤组织中ERK5、MMP-9的阳性表达组患者生存时间均短于阴性表达组(均P<0.05)。单因素Cox回归分析发现,肿瘤大小、Enneking分期、转移、ERK5和MMP-9阳性表达与患者总生存时间相关(均P<0.05);多因素分析证实Enneking分期、转移、ERK5和MMP-9蛋白的阳性表达可作为骨肉瘤患者独立的预后因子(均P<0.05)。结论:ERK5和MMP-9在骨肉瘤组织中高表达,与骨肉瘤的病理特征及预后相关;二者可能在骨肉瘤发生发展的过程中起着重要作用。Objective：To investigate the extracellular signal-regulated kinase 5（ERK5） and matrix metallo proteinase-9（MMP-9） expression levels in osteosarcoma tissues and their clinical significance.Methods：The ERK5 and MMP-9 expression levels in 71 specimens of osteosarcoma tissue and 40 specimens of normal bone tissue were detected by immunohistochemistry.The relationship between ERK5 and MMP-9 expression levels,their clinical characteristics,and prognosis of patients with osteosarcoma were analyzed.Results：The positive expression of ERK5 and MMP-9 in osteosarcoma tissues was 85.9%（61/71） and 74.65%（53/71）,respectively,which were significantly higher than those in normal bone tissues at 12.5%（5/40） and 10.0%（4/40）（all P〈0.05）.The positive expression of ERK5 and MMP-9 was associated with Enneking stage and metastasis（all P〈0.05）.Kaplan-Meier analysis showed that the survival duration of patients with positive ERK5 and MMP-9 expression levels was shorter than those of the patients in the negative expression groups（all P〈0.05）.Univariate analysis of COX proportional hazards regression model revealed that tumor size,Enneking stage,metastasis,and positive ERK5 and MMP-9 expression levels are relevant to the overall survival of patients with osteosarcoma（all P〈0.05）.Multivariate analysis of COX proportional hazards regression model confirmed that Enneking stage,metastasis,and positive ERK5 and MMP-9 expression levels can act as independent prognostic factors for osteosarcoma patients（all P〈0.05）.Conclusion：The ERK5 and MMP-9 expression levels are high in osteosarcoma tissues and are related to the clinical characteristics and prognosis of patients with osteosarcoma.Thus,ERK5 and MMP-9 expression levels may play important roles in osteosarcoma development and progression.

关 键 词：骨肉瘤 细胞外信号调节激酶5 基质金属蛋白酶-9 免疫组织化学 预后 

分 类 号：R738.1[医药卫生—肿瘤]