miR-132表达上调对局灶性脑缺血大鼠皮层Ang-1／Tie2表达的影响  

Effect of up-regulation of miR-132 on expressions of angiopoietin-1/endothelium-specific tyrosine kinase receptor 2 in focal cerebral ischemia reperfusion injury of rats

作　　者：陈澎[1] 陈传磊[1] 侯爱武[1] 高茜[1] 张仕娟[1] 孙锡波[1] 李炳选[1] 牛建一[1] 

机构地区：[1]潍坊医学院附属益都中心医院神经内一科,潍坊262500

出　　处：《中华神经医学杂志》2017年第8期772-776,共5页Chinese Journal of Neuromedicine

基　　金：2016年度潍坊市卫生计生委科研计划项目（2016wsjs050）

摘　　要：目的探讨miR-132表达上调对局灶性脑缺血大鼠皮层血管生成素1（Ang^-1）及其内皮特异性酪氨酸激酶受体（Tie2）表达的影响。方法40只成年健康雄性SD大鼠按随机数字表法分为假手术组、脑缺血组、miR-132模拟物组和阴性对照组，每组10只。后3组按照改良Longa线栓法制备成大脑中动脉闭塞模型，其中miR-132模拟物组和阴性对照组于脑缺血5min时分别将miR-132模拟物15μg和阴性对照物15μg经侧脑室注入。缺血后24h取脑组织，采用2，3，5．氯化三苯基四氮唑（TTC）染色计算各组大鼠脑梗死体积，采用实时荧光定量PCR检测缺血侧皮层组织miR-132、Ang^-1、Tie2 mRNA表达，采用Western blotting检测缺血侧皮层组织Ang^-1、Tie2、CD31、血管内皮生长因子（VEGF）蛋白表达。结果miR-132模拟物组脑梗死体积[（27．92±3．05）mm^3]显著低于脑缺血组和阴性对照组[（51．34±2．86）mm^3和（50．46±2．571mm^3]，差异均有统计学意义（P〈0．05）。miR-132模拟物组缺血侧皮层组织miR-132、Ang—1、Tie2mRNA相对表达量及Ang^-1、Tie2、CD31、VEGF蛋白相对表达量均明显高于阴性对照组、脑缺血组和假手术组，且阴性对照组和脑缺血组均明显高于假手术组，差异均有统计学意义（P〈0．05）。结论上调miR-132表达可改善脑缺血大鼠脑组织缺血性损伤，其机制可能与促进Ang^-1／Tie2表达进而促进缺血脑组织血管再生有关．Objective To investigate the effect of up-regulation of miR-132 on expressions of angiopoietin-1 （Ang^-1）/endothelium-specific tyrosine kinase receptor 2 （Tie2） in focal cerebral ischemia reperfusion injury of rats. Methods Forty adult healthy SD rats were randomly divided into sham-operated group, cerebral ischemia group, miR-132 mimic group and negative control group （n=10）. The models of middle cerebral artery occlusion in the later three groups were established by using modified Longa suture method. Rats in the miR-132 mimic group and negative control group were injected miR-132 mimic 15 tzg and negative control 15 p^g via paracele. Rats in each group were sacrificed 24 h after ischemia, and the brain tissues were collected; the total infarct volumes were calculated by TTC staining. The mRNA expressions ofmiR-132, Ang^-1 and Tie2 in ischemic cerebral cortex tissues were detected by real-time fluorescence quantitative PCR. The protein expressions of Ang^-1, Tie2, CD31 and vessel endothelial growth factor （VEGF） in ischemic cerebral cortex tissues were detected by Western blotting. Results The total infarct volume in the miR-132 mimic group was （27.92±3.05） mm^3, which was significantly smaller than that in the cerebral ischemia group and negative control group （[51.34±2.86] mm^3 and [50.46±2.57] mm^3, P〈0.05）. The relative mRNA expression levels of miR-132, A rig^-1, Tie2, and the protein expression levels of Ang^-1, Tie2, CD31, VEGF in the ischemiccerebral cortex tissues of the miR-132 mimic group were significantly higher than those in the negative control group, cerebral ischemia group and sham-operated group （P〈0.05）; and those in the negative control group and cerebral ischemia group were significantly higher than those in the sham-operated group （P〈0.05）. Conclusion Up-regulation of miR-132 expression could improve the ischemic states of ischemic stroke in rats, which might be related to Ang^-1/Tie2 increased expressions to promote angiogenesis in ischemic brain t

关 键 词：miR-132 脑缺血 血管生成素1 内皮特异性酪氨酸激酶受体 

分 类 号：R743.3[医药卫生—神经病学与精神病学]