BDNF联合chABC治疗对脊髓损伤大鼠神经传导通路的修复研究  

Effect of combined treatment of brain-derived neurotrophic factor with chondroitinase ABC on nerve transduction pathway in rats after spinal cord injury

作　　者：李晓彬[1] 刘涛[1] 周俊锋[1] 范磊[1] 

机构地区：[1]河南省人民医院急诊创伤外科,郑州450003

出　　处：《中华神经医学杂志》2017年第8期785-789,共5页Chinese Journal of Neuromedicine

基　　金：河南省基础与前沿技术研究计划（122300410195）

摘　　要：目的研究脑源性神经营养因子（BDNF）联合硫酸软骨素酶ABC（chABC）治疗对脊髓损伤大鼠神经传导通路的修复作用。方法雌性SD大鼠150只按随机数字表法分为假手术组、生理盐水治疗组、BDNF治疗组、chABC治疗组、BDNF联合chABC治疗组，每组30只。后4组大鼠制作脊髓损伤模型，术后3d分别于损伤中心注射10μL生理盐水、20μL BDNF、6μL chABC、6μL ch ABC＋20μL BDNF，连续治疗60d，假手术组大鼠不做任何治疗。造模后1d、7d、14d、28d、60d对5组大鼠进行BBB运动功能评分检测和体感诱发电位（SEP）、运动诱发电位（MEP）监测，观察大鼠脊髓神经传导功能的恢复情况。结果伤后28、60d，生理盐水治疗组、BDNF治疗组和chABC治疗组、BDNF联合chABC治疗组大鼠BBB评分逐渐增高，差异均有统计学意义（P〈0．05）；伤后14d，BDNF联合chABC治疗组大鼠SEPN1波潜伏期[（27．16±1．09）s]低于生理盐水治疗组[（31．25±1．54）s]，差异有统计学意义（P〈0．05）；伤后28d、60d，生理盐水治疗组、BDNF治疗组和chABC治疗组、BDNF联合chABC治疗组大鼠SEPN1波潜伏期逐渐降低，差异均有统计学意义（P〈0．05）；伤后14d、28d、60d，生理盐水治疗组、BDNF治疗组和chABC治疗组、BDNF联合chABC治疗组大鼠MEPN1波潜伏期逐渐降低，差异均有统计学意义（P〈0．05）。结论BDNF联合chABC治疗对脊髓损伤具有神经保护和神经营养作用，可加快脊髓损伤大鼠神经传导功能的恢复。Objective To investigate the effect of combined treatment of brain-derived neurotrophic factor （BDNF） with chondroitinase ABC （chABC） on repair of spinal cord injury in rats via nerve transduction pathway. Methods One hundred and fifty female SD rats were randomly divided into sham-operated group, normal saline （NS） treatment group, BDNF treatment group, chABC treatment group, and BDNF and chABC treatment group （n=30）. The spinal cord injury models of rats in the later 4 groups were established successfully, and 10 μL NS, 20μL BDNF, 6 μL chABC and 6 μL chABC±20 μL BDNF were given to the injury tissues 3 d after injury for a consecutive 60 d. Basso-Beattle-Bresnahan （BBB） scale was performed, and matosensory evoked potential （SEP） and motor evoked potential （MEP） were monitored; the reparation of nerve transduction pathway was observed one, 7, 14, 28 and 60 d after spinal cord injury. Results The BBB scale scores increased accordingly in the NS treatment group, BDNF treatment group, chABC treatment group, and BDNF and chABC treatment group 28 and 60 d after injury, with significant differences between each two groups （P〈0.05）. The latency of SEP N1 wave in the BDNF and chABC treatment group （[27.16±1.09] s） was significantly lower than that in the NS treatment group （[31.256±1.54] s） 14 d after injury （P〈0.05）. The latency of SEP N1 wave decreased accordingly in the NS treatment group, BDNF treatment group, chABC treatment group, and BDNF and chABC treatment group 28 and 60 d after injury, with significant differences between each two groups （P〈0.05）. The latency of MEP N1 wave decreased accordingly in the NS treatment group, BDNF treatment group, chABC treatment group, and BDNF and chABC treatment group 14, 28 and 60 d after injury, with significant differences between each two groups （P〈 0.05）. Conclusion Combined treatment of BDNF with chABC has neurotropic and neuroprotective effects on spinal cord injury, which could help the recovery of nerv

关 键 词：脊髓损伤 脑源性神经营养因子 硫酸软骨素酶ABC 诱发电位 

分 类 号：R651.2[医药卫生—外科学]