聚类分析和主成分分析法研究金蝉花不同部位的HPLC指纹图谱  

HPLC fingerprint of Cordyceps cicadae from different parts by cluster analysis and principal component analysis

作　　者：李玲[1] 梁瀚文[1] 吴卓娜[1] 张忠亮 张莉莉[1] 王玉芹 张彤[1] 王冰[1] 

机构地区：[1]上海中医药大学,上海201203 [2]浙江泛亚生物医药股份有限公司,浙江平湖314200

出　　处：《时珍国医国药》2017年第7期1537-1541,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81303233)

摘　　要：目的建立金蝉花不同部位的HPLC指纹图谱,并进行聚类分析和主成分分析。方法采用WELCH Welchrom C18色谱柱;流动相为水-甲醇,梯度洗脱;体积流量1.0 ml·min^(-1);检测波长260 nm;柱温25℃。结果金蝉花菌核HPLC指纹图谱中有10个共有峰,孢梗束HPLC指纹图谱中有6个共有峰,相似度均大于0.90,其中5个峰被确认为胞嘧啶、尿苷、肌苷。结论 20份样品可被聚成2类,分别为菌核和孢梗束,并且2个主成分的累计方差贡献率为86.8%。Objective To establish an HPLC fingerprint of Cordyceps cicadae from different parts by cluster analysis and principal component analysis. Methods The analysis was performed on WELCH Welchrom C18 column,mobile phase was water-acetonitrile with gradient elution,flow rate was 1. 0 ml·min-1,detection wavelength was set at 260 nm,and column temperature was maintained at 25 ℃. Results There were ten common peaks in the HPLC fingerprints of sclerotium of Cordyceps cicadae and six common peaks in the HPLC fingerprints of synnemata of Cordyceps cicadae. The similarities of fingerprints were greater than 0. 90 with good separation of each chromatographic peak,among of them,Cytosine,Uridine,Inosine,Guanosine and Adenosine were identified. Conclusion 20 batches samples can be classified into two groups,and 2 principal components make up 86. 8% of the total variance.

关 键 词：金蝉花 HPLC指纹图谱 聚类分析 主成分分析 菌核 孢梗束 

分 类 号：R284.1[医药卫生—中药学]