金铁锁糖基转移酶UGT71G1的克隆与生物信息学分析  

Cloning and Bioinformatics of UGT71G1 genes of Psammosilene tunicoides

作　　者：李媛[1] 李国栋[1] 张爱丽[1] 钱子刚[1] 

机构地区：[1]云南中医学院/中药材优良种苗繁育工程中心,云南昆明650500

出　　处：《时珍国医国药》2017年第7期1751-1753,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260609);云南省教育厅重点项目(No.2015119);云南省教育厅科学研究基金项目(No.2015J101)

摘　　要：目的克隆金铁锁糖基转移酶全长基因,并进行生物信息学分析。方法根据转录组数据,反转录获得全长c DNA,通过生物信息学软件对该基因蛋白特性及系统发育树进行分析。结果克隆得到c DNA全长序列,命名为UGT71G1,该c DNA全序列长1402 bp,包含一条1107 bp完整开放阅读框,编码368个氨基酸,预测相对分子质量为41.05KD,等电点6.03,在155位到336位置处存在高度保守UDPGT结构功能域,经多重序列比对与同科植物香石竹具有很高的同源性。结论成功克隆、分析了金铁锁UGT71G1基因,为金铁锁有效成分合成关键酶基因及调控机制研究提供基础。Objective To clone the glycosyltransferase gene（ UGT） in Psammosilene tunicoides,and to analyze the bioinformation of UGT71G1. Methods c DNA was reversely transcriped according to the transcriptome sequencing. The protein characteristics was analyzed and the phylogenetic tree of UGT was constructed using the bioinformatics. Results The 1402 bp sequence in P.tunicoides named UGT71G1 was obtained,which has a 1107 bp ORF,encoding 368 amino acids. The protein molecular weight was 41. 05 KD,with the isoelectric point of 6. 03. The highly conserved functional domains of glycosyltransferase gene was found from 155 sites to 336 sites. The UGT in was most similar with Dianthus caryophyllus by NCBI blast. Conclusion The UGT in P.tunicoides was successfully cloned and analyzed,which provides the foundation for composite and regulation mechanism of the active ingredients in P. tunicoides.

关 键 词：金铁锁 糖基转移酶 生物信息 

分 类 号：R284[医药卫生—中药学]