电针干预对D-半乳糖致衰大鼠术后认知功能障碍及海马AngⅡ/AT1R的影响  

Effects of electroacupuncture on postoperative cognitive dysfunction and AngⅡ/AT1R in the hippocampus in aging rats induced by D-galactose

作　　者：冯培培[1] 刘喆[1] 王昕[2] 王文娟[1] 王改梅[3] 黎娜[1] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053 [2]浙江省杭州市余杭区中医院 [3]浙江省中山医院

出　　处：《中国针灸》2017年第8期863-868,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81273822;国家重点基础研究发展计划("973"计划)项目:2013 CB 531906;浙江省中医药科技计划项目:2012 ZB 074;浙江省高校中医学重中之重一级学科(针灸推拿学)研究生创新计划项目:2015203

摘　　要：目的:观察电针对D-半乳糖致衰大鼠部分肝叶切除术后认知功能障碍(POCD)及对海马血管紧张素Ⅱ(AngⅡ)和1型受体(AT1R)的影响,探讨电针干预对术后认知功能影响的可能作用机制。方法:80只雄性SD大鼠随机分为青年对照组(10只),D-半乳糖致衰(D-Galactose induced aged,Da)组(10只),Da+肝叶切除术组(30只,再随机分为1 d、3 d、7 d组3个亚组,10只/亚组)和电针组(30只,再随机分为1 d、3 d、7 d组3个亚组,10只/亚组)。电针组穴取"百会""大椎",给予连续波,15 Hz,1 m A干预治疗,各亚组分别干预1 d、3 d、7 d,其余各组只抓取固定,每天1次。采用Y迷宫检测大鼠认知行为学改变,ELISA法检测海马AngⅡ含量,荧光定量PCR(RT-PCR)和免疫组化法分别检测海马AT1R m RNA及其阳性表达。结果:Da组大鼠主动回避次数显著少于青年对照组(P<0.05),其海马AT1R m RNA和阳性细胞百分比均显著多于青年对照组(均P<0.01);与Da组比较,Da+肝叶切除术1 d、3 d和7 d各亚组和电针1 d、3 d和7 d各亚组大鼠主动回避次数均较少(均P<0.01),AngⅡ水平、AT1R m RNA表达和阳性百分比均增加(P<0.05,P<0.01);电针组各亚组大鼠主动回避次数均显著多于同时间点Da+肝叶切除术各亚组(P<0.05,P<0.01),其相应海马AngⅡ水平、AT1R m RNA表达和AT1R阳性百分比均显著少于同时间点Da+肝叶切除术组(P<0.05,P<0.01)。结论:电针"百会""大椎"穴可改善Da致衰大鼠部分肝叶切除后认知功能障碍行为,其作用机制可能与抑制海马AngⅡ、AT1R阳性表达及AT1R m RNA有关。Objective To observe the effects of electroacupuncture（EA） on postoperative cognitive dysfunction（POCD） and AngⅡ/AT1 R in the hippocampus in D-galactose-induced aging rats which received hepalobectomy, and to explore the possible mechanism of EA on POCD. Methods Eighty male Sprague-Dawley rats were randomly divided into a young control group（10 rats）, a D-Galactose-induced aged（Da） group（10 rats）, a Da＋hepatolobectomy group（30 rats） and an EA group（30 rats）. The rats in the Da＋hepatolobectomy group and EA group were further randomly divided into a 1 d subgroup, 3 d subgroup and a 7 d subgroup, 10 rats in each subgroup. The rats in the EA group were treated with EA at ＂Baihui＂（GV 20） and ＂Dazhui＂（GV 14） with continuous wave（15 Hz in frequency and 1 m A in intensity）, and rats in each subgroup were treated for 1 d,3 d and 7 d, respectively. The rats in the remaining groups were treated with immobilization, once a day. The Y-maze was used to observe the behavior change of rats, and ELISA was applied to measure the level of hippocampal AngⅡ, and RT-PCR and immunohistochemistry method were performed to detect AT1 R m RNA expressions and AT1 R positive expression in the hippocampus. Results The number of rat initiative avoidance in the Da group was significantly less than that in the young control group（P〈0.05）, and the m RNA expression and positive percentage of AT1 R in the hippocampus in the Da group were significantly higher than those in the young control group（both P〈0.01）. Compared with the Da group, the number of ratinitiative avoidance in each subgroup of Da＋hepatolobectomy group and EA group were significantly reduced（all P〈0.01）,and the expression of AngⅡ, AT1 R m RNA and AT1 R positive cells percentage in the hippocampus were significantly increased（P〈0.05, P〈0.01）. The number of rat initiative avoidance in each subgroup of EA group was higher than that in the subgroup of Da＋hepatolobectomy group（P〈0.05, P〈0.01）;

关 键 词：电针 术后认知功能障碍 AngⅡ AT1R D-半乳糖致衰大鼠 海马 

分 类 号：R245.97[医药卫生—针灸推拿学]