猪A-FABP基因的克隆及其组织差异性表达  

Cloning and differential expression of A-FABP gene in tissues of swine

作　　者：李晓玲[1] 崔小荣[1] 窦成利[1] 于光辉[1] 张廷荣[1] 孙金海[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109

出　　处：《黑龙江畜牧兽医》2017年第8期106-110,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：转基因生物新品种培育科技重大专项项目(2016ZX08006-003);山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-13)

摘　　要：为了进行猪A-FABP基因的克隆及不同器官组织中的差异表达,试验采用RT-PCR方法从长白猪背最长肌中克隆A-FABP基因的cDNA序列并进行测序比对,分别与人、牛、山羊、家鼠及鸡进行同源性比较,应用半定量RT-PCR方法检测猪心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌中A-FABP基因的表达。结果表明:成功克隆得到A-FABP基因的cDNA序列,且比对结果为100%,该序列与人、牛、羊的同源性分别为90.98%、89.97%、89.97%,与鸡的同源性为73.68%。半定量RT-PCR结果为A-FABP基因在心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌器官组织中均有表达,其中背最长肌和腿肌中A-FABP基因的表达量最高,肺脏内的表达量最低,A-FABP基因在动物进化中具有高度保守性,该基因在猪不同器官组织中的表达具有差异性。In order to investigate the cloning and differential mRNA expression of A - FABP gene in different tissues of swine,in this experiment the cDNA of A - FABP gene from longissimus dorsi muscle of swine was cloned and sequenced by RT - PCR, and was aligned in comparison with human, cattle, goat, house mouse and chicken respectively. The expression of A - FABP mRNA in different tissues （ heart, liver, spleen, lung, kidney, longissimus dorsi muscle and leg muscle） was detected by using semi - quantitative RT - PCR. The results showed that the cDNA of A - FABP gene was successfully cloned, and was in 100% homology with the standard sequence in NCBI database. The A - FABP gene shared about 90.98% ,89.97% ,89.97% homology with people,cattle and goat respectively,while sharing about 73.68% with chicken. The results of semi - quantitative RT - PCR indicated that pig A - FABP mRNA was expressed in all seven tissues （ heart,liver, spleen,lung, kidney, longissimus dorsi muscle and leg muscle） ,with the highest levels were in lnngissimus dorsi muscle and leg muscle and the lowest was in lung. A - FABP gene could be highly conservative during the evolution of swine. The expression level of A - FABP mRNA was variant in different tissues.

关 键 词：猪 A-FABP基因 克隆 同源性 组织表达 

分 类 号：Q785[生物学—分子生物学] S828[农业科学—畜牧学]