猪伪狂犬病gB抗体快速检测试纸条的研制和评价  

Development and evaluation of a rapid test strip for gB antibody detection of pseudorabies virus

作　　者：邱荣超[1] 韩占兵[1] 刘永祥[1] 唐国盘[1] 李文刚[1] 

机构地区：[1]河南牧业经济学院,河南郑州450046

出　　处：《中国兽医学报》2017年第8期1463-1467,共5页Chinese Journal of Veterinary Science

基　　金：河南省科技攻关计划重点资助项目(152102110093)

摘　　要：为了建立一种猪伪狂犬病gB抗体检测试纸,为养猪业基层工作人员提供一种快速简便的狂犬病免疫抗体评价方法。胶体金标记gB蛋白作为探针,SPA和猪抗伪狂犬病病毒多抗IgG分别作为检测线和质控线,建立了猪伪狂犬病gB抗体检测试纸。猪伪狂犬病质控血清和疫苗免疫猪血清用来评价其特异性,敏感性以及与IDEXX PRV gB ELISA试剂盒的符合率试验。试验结果显示,该试纸条具有较高的特异性和敏感性,与IDEXX PRV gB ELISA试剂盒的符合率为91.04%;该免疫层析试纸条具有快速(5min)、特异、敏感等优点,不需要专门的仪器设备和专业技术人员,适合于田间推广应用。To evaluate the gB antibody level of pseudorabies virus （PRV） in swine,a rapid test strip was developed. In the strip, the expressed protein of gB was labeled with colloidal gold, the staphy- lococcal protein A （SPA） and swine anti PRV antibody were blotted on the nitrocellulose mem- brane for the test and control lines, respectively. The specificity and sensitivity of the strip were detected with standard positive,negative and immunized sera of PRV, the results indicated that the strip was high specificity and sensitivity. Field swine serum samples were tested by the new strip and commercial IDEXX PRV gB ELISA kit, simultaneously. The agreement rate of the two meth- ods was 91.04%. Furthermore,the dipstick assay based on the strip is rapid （5 min） ,sensitive and easy to perform. This suggests that the new strip is an acceptable alternative for field diagnosis.

关 键 词：伪狂犬病病毒 试纸条 ELISA 

分 类 号：S852.65[农业科学—基础兽医学]