SE感染对雏鸡空回肠组织中TLR4信号转导通路及pIgR的影响  

Expression of TLR4 signal pathway and pIgR in SE-induced inflammation response

作　　者：李德军[1] 周晓玲 刘运枫[1] 宋雪[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]黑龙江省动物疫病预防与控制中心,黑龙江哈尔滨150069

出　　处：《中国兽医学报》2017年第8期1545-1548,共4页Chinese Journal of Veterinary Science

基　　金：黑龙江省博士后启动基金资助项目(LBH-Z11239);东北农业大学博士启动基金资助项目(2012RCB22)

摘　　要：为了研究肠炎沙门菌(SE)对TLR4信号通路调节雏鸡空肠和回肠组织中多聚免疫球蛋白受体(pIgR)的影响,本试验以7日龄海兰褐雏鸡的空肠和回肠组织为主要研究对象,通过口服SE的方式感染雏鸡,于感染后的1,3,7和14d,检测雏鸡的空肠和回肠组织中TLR4信号通路下游因子MyD88、TRAF6、NF-B和pIgR mRNA表达。结果表明:雏鸡感染SE后,TLR4信号通路被激活,且空肠、回肠组织中pIgR mRNA的表达量升高(P<0.01),同时上调空肠和回肠组织中pIgR的蛋白水平。证实SE能够激活空肠和回肠黏膜细胞表面的TLR4信号通路并上调pIgR的表达,进而增强雏鸡的肠黏膜免疫。To observe the effect of pIgR on Salmonella Enteritidis induced inflammation in jeju- num and ileum in Chicken, 7 day Hyline chickens were taken orally with Salmonella Enteritidis （SE） and killed after 1 d,3 d,7 d and 14 d. The mRNA expression of Toll-like receptor 4（TLR4）, MyD88,TRAF6,NF-κB and pIgR was detected by real-time RT-PCR and pIgR protein level was detected by Western blot. The results showed that TLR4 signaling pathway was activated and mR- NA level of the pIgR in jejunum and ileum was enhanced （P〈0.01） and protein level of the pIgR in jejunum and ileum was up-regulated by SE. The study proved that TLR4 signal pathway on mu- cosal cell surface of jejunum and ileum was activated and expression of pIgR was up-regulated and gut mucosal immunity of chicken was strenghtened.

关 键 词：肠炎沙门菌 Toll受体4 空肠 回肠 pIgR 

分 类 号：S852.61[农业科学—基础兽医学]