民猪FoxN1基因的表达规律及对低温应答反应  

Expression pattern of FoxN1 gene and response to low temperature in Min pig

作　　者：张冬杰[1] 李苓[2] 王鑫鑫[2] 汪亮[1] 杨国伟[1] 刘娣 

机构地区：[1]黑龙江省农业科学院畜牧研究所,黑龙江哈尔滨150086 [2]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《中国兽医学报》2017年第8期1589-1593,1604,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31301955);黑龙江省自然科学基金资助项目(C201312);国家生猪产业体系资助项目(CARS-36)

摘　　要：胸腺是哺乳动物的一个重要免疫器官,FoxN1在调节胸腺上皮细胞发育中起着重要作用。猪既是一种肉用型家养动物,同时也是一种新型的实验动物模型。为了更好的了解和掌握猪自身免疫的发展特点,本试验采用Realtime PCR结合Western blot技术对2,4,6,8月龄民猪胸腺内FoxN1的表达变化进行了检测,同时也分析了冷应激后FoxN1基因在耐冷型民猪和非耐冷型大白猪胸腺内的表达变化情况。结果表明,FoxN1基因在4月龄民猪胸腺内的表达水平最高,从6月龄开始出现下降,与已知人、小鼠的FoxN1表达变化趋于一致。冷应激会促进民猪胸腺FoxN1基因的转录与表达,但对大白猪无效。Thymus is an important immune organ in mammals. FoxN1 plays an important role in regulating the development of thymic epithelial cells. Pig is a domestic animal of meat type,and is also a new kind of experimental animal model. In order to better understand the development char- acteristics of pig own immunity, the expression pattern of Minzhu FoxN1 in 2 month, 4 month, 6 month and 8 month thymus were detected by real-time PCR and Western-blot. At the same time, the changes of FoxN1 expression in Minzhu and Large white pig after cold stress were also detected. The results showed that FoxN1 expression level reached the highest level in 4 month,and declined in 6 month. This expression pattern is consistent with human and mouse. Cold stress could rise the expression level of Minzhu FoxNl,while have no effect on the Large white pig.

关 键 词：民猪 大白猪 胸腺 FoxN1基因 

分 类 号：S852.2[农业科学—基础兽医学]