心痛泰通过降低Lp-PLA2、IL-6、hs-CRP、LOX-1水平减轻兔动脉粥样硬化炎症反应  

Xin Tong-tai reduces inflammation of atherosclerosis by lowering the levels of Lp-PLA2,IL-6,hs-CRP and LOX-1 in rabbits

作　　者：易琼[1] 李雅[2] 郭志华[2] 乐金海 任欣[3] 申思[3] 

机构地区：[1]湖南中医药大学第一附属医院重症医学科,湖南省长沙市410007 [2]湖南中医药大学药学院,湖南省长沙市410208 [3]湖南中医药大学第一附属医院老年病科,湖南省长沙市410007

出　　处：《中国动脉硬化杂志》2017年第8期796-799,共4页Chinese Journal of Arteriosclerosis

基　　金：湖南省中医药科研项目(201516)

摘　　要：目的研究心痛泰对动脉粥样硬化兔血清脂蛋白相关磷脂酶A2(Lp-PLA2)、白细胞介素6(IL-6)以及主动脉组织高敏C反应蛋白(hs-CRP)和血凝素样氧化型低密度脂蛋白受体1(LOX-1)的影响。方法 120只日本大耳白兔随机分为空白组、模型组、心痛泰低剂量组、中剂量组、高剂量组和瑞舒伐他汀组。采用前瞻性研究,造模的同时予以相应药物灌胃。60天后采血,测定血清Lp-PLA2和IL-6水平;免疫组织化学法检测胸主动脉hs-CRP和LOX-1含量。结果心痛泰低剂量组、中剂量组和高剂量组血清Lp-PLA2和IL-6水平比模型组低(P<0.01);与心痛泰低剂量组比较,心痛泰中剂量组和高剂量组、瑞舒伐他汀组血清Lp-PLA2和IL-6水平降低(P<0.01或P<0.05)。心痛泰中剂量组和高剂量组的hs-CRP和LOX-1表达水平比模型组低(P<0.01);与心痛泰低剂量组比较,心痛泰中剂量组和高剂量组、瑞舒伐他汀组的hs-CRP和LOX-1表达水平降低(P<0.05)。免疫组织化学染色显示,模型组主动脉内膜hs-CRP和LOX-1大量表达;与模型组比较,心痛泰低剂量组、中剂量组、高剂量组和瑞舒伐他汀组主动脉内膜hs-CRP和LOX-1表达减轻。结论心痛泰能降低动脉粥样硬化兔Lp-PLA2、IL-6、hs-CRP、LOX-1的水平,从而达到抗动脉粥样硬化炎症反应的作用。Aim To study the effects of Xin Tong-tai（ XTT） on serum lipoprotein-associated phospholipase A2（ Lp-PLA2） and interleukin 6（ IL-6）,and aortic tissue high-sensitivity C-reactive protein（ hs-CRP） and lectin-like oxidized low density lipoprotein receptor-1（ LOX-1） in atherosclerotic rabbits. Methods A total of 120 healthy Japanese rabbits were randomly divided into control group,model group,low-dose XTT group,middle-dose XTT group,high-dose XTT group and rosuvastatin group. In this prospective study,the drug intervention groups were treated with the corresponding drugs at the same time. After 60 days,serum levels of Lp-PLA2 and IL-6 were measured; The contents of hsCRP and LOX-1 in thoracic aorta were detected by immunohistochemistry method. Results The serum levels of LpPLA2 and IL-6 in low-dose XTT group,middle-dose XTT group and high-dose XTT group were lower than those in model group（ P〈0.01）. Compared with low-dose XTT group,the serum levels of Lp-PLA2 and IL-6 were decreased in middledose XTT group,high-dose XTT group and rosuvastatin group（ P〈0.01 or P〈0.05）. The expression levels of hs-CRP and LOX-1 in middle-dose XTT group and high-dose XTT group were lower than those in model group（ P〈0.01）. Compared with low-dose XTT group,the expression levels of hs-CRP and LOX-1 were decreased in middle-dose XTT group,highdose XTT group and rosuvastatin group（ P〈0.05）. Immunohistochemical staining showed that in model group,there was a large amount of hs-CRP and LOX-1 expression in aortic intima; Compared with model group,the expressions of hs-CRP and LOX-1 in the aorta were reduced in low-dose XTT group,middle-dose XTT group,high-dose XTT group and rosuvastatin group. Conclusion Xin Tong-tai can decrease the levels of Lp-PLA2,IL-6,hs-CRP and LOX-1 in atherosclerotic rabbits,so as to achieve the effect of reducing atherosclerosis inflammatory reaction.

关 键 词：心痛泰 动脉粥样硬化 脂蛋白相关磷脂酶A2 白细胞介素6 高敏C反应蛋白 血凝素样氧化型低密度脂蛋白受体1 兔 

分 类 号：R285[医药卫生—中药学]