知柏地黄汤对解脲脲原体感染大鼠生精细胞线粒体细胞色素氧化酶的影响  

Effects of Zhibai Dihuang Decoction on mitochondrial cytochrome oxidase in the spermatogenic cells of rats with ureaplasma urealyticum infection

作　　者：郭军华[1,2] 李迎秋[1] 郭炫佐[1] 刘朝圣[1] 何清湖[1] 

机构地区：[1]湖南中医药大学研究生院,湖南长沙410007 [2]盐城市中医院男科,江苏盐城224002

出　　处：《中华男科学杂志》2017年第8期722-727,共6页National Journal of Andrology

基　　金：国家自然科学基金(81373641);湖南省科技厅课题资助项目(2011SK3104);长沙市科技局课题资助项目(K1301014-31);湖南省科技计划项目(2012SK31387)~~

摘　　要：目的:探讨解脲脲原体(UU)感染对大鼠生精细胞线粒体细胞色素氧化酶(COX)的影响以及知柏地黄汤对其的干预作用。方法:4~5月龄SD雄性大鼠40只,随机抽取30只,经膀胱接种UU悬液,建立UU睾丸感染动物模型。剩余10只同步注射生理盐水作为正常组,于接种后的第7天取睾丸穿刺液运用培养法检测UU,判断造模成功否。造模成功后,UU感染模型大鼠再随机分成模型组、知柏地黄汤组、阿奇霉素组。知柏地黄汤组给药量为1 g/(kg·d),阿奇霉素组给药量为0.105 mg/(kg·d),正常组、模型组给予相同剂量的生理盐水。以上各组均灌胃给药,每日1次,连续给药21 d(1 ml/d)。采用颈椎脱臼法处死大鼠,取附睾组织,采用机械分离法分离精子细胞,采用彩色精子动态检测系统检测精子运动参数;取睾丸组织,采用免疫组化法(DAB法)检测COX活性,RT-PCR法检测COXⅠ、ⅡmRNA表达。结果:模型组大鼠a级精子,b级精子,直线速度,平均速度,COXⅠ、ⅡmRNA表达均明显低于正常组(P<0.05,P<0.01)。经治疗后,知柏地黄汤组和阿奇霉素组a级精子、b级精子、直线速度、曲线速度、平均速度水平及COXⅠmRNA表达均提高,与模型组比较,差异有统计学意义(P<0.05,P<0.01),分别为a级精子[(1.11±0.30)、(0.60±0.19)、(0.07±0.03)%]、b级精子[(2.40±0.59)、(1.32±0.27)、(0.35±0.13)%]、直线速度[(12.11±1.62)、(11.47±1.21)、(6.78±1.05)μm/s]、曲线速度[(54.30±2.35)、(45.75±1.64)、(38.10±7.65)μm/s]、平均速度[(18.40±1.27)、(16.69±1.02)、(10.02±1.08)μm/s]、COXⅠmRNA[(1.86±0.30)、(1.74±0.17)、(0.93±0.10)%],而两组均不能提高COX活性和COXⅡmRNA表达,知柏地黄汤组在提高a级精子、b级精子、曲线速度、平均速度方面效果优于阿奇霉素组,差异有统计学意义(P<0.05,P<0.01)。结论:UU感染能够降低大鼠a级精子,b级精子,直线速度,平均速度水平,COXⅠ、ⅡmRNA表达。知柏地黄汤和阿奇霉素对其�Objective： To explore the effects of Zhibai Dihuang Decoction （ZDD） on mitochondrial cytochrome oxidase （COX） in the spermatogenic cells of rats with ureaplasma urealyticum （UU） infection. Methods ： From forty 4 - 5 months old SD rats, 30 were randomly selected for the establishment of the model of testicular UU infection by inoculating the bladder with UU suspension and the other 10 injected with normal saline as controls （group A）. At 7 days after inoculation, the rat models of testicular UU infection were treated orally with normal saline （group B）, ZDD at 1 g per kg of the body weight per day （group C）, and azithromycin at 0. 105 g per kg of the body weight per day （ group D） , respectively, once daily for 21 days. Then all the animals were sacrificed and the epididymal and testicular tissues collected for examination of sperm motility with the color sperm dynamic detection system, meas- urement of the COX activity with the immunohistochemical DAB method, and determination of the mRNA expressions of COX I and COX II by RT-PCR. Results： Compared with group A, group B showed significant decreases in such sperm parameters as grade a sperm （[1.03 ± 0.09] vs [0.07 ± 0.03] %, P〈0.01）, grade b sperm （[2.07 ± 0.52] vs [0.35 ± 0.13] %, P〈0.01）, straight line velocity （VSL） （ [ 10.95 ± 0.98 ] vs [ 6.78 ± 1.05 ] μm/s, P 〈 0.01 ）, curvilinear velocity （VCL） （ [ 42.03± 1.35] vs [38.10 ± 7.65] μm/s, P〉0.05）, average path velocity （VAP） （[16.22 ± 1.52] vs [10.05 ± 1.80] μm/s, P〈 0.01）, and the mRNA expressions of COX I （[2.25 ±0.24] vs [0.93 ± 0.10] %, P〈0. 01） and II （[6.72 ± 0.37] vs [2.95 ±0. 78 ] % , P 〈0.01 ）. After treatment, all the parameters were remarkably increased in groups C and D （ grade a sperm： [1.11 ± 0.30] and [0.60 ± 0.19] %; grade b sperm： [2.40 ± 0.59] and [1.32 ± 0.27] %; VSL： [12.11 ± 1.62] and [11.47 ± 1.21] μm/s; VCL： [54.30 ± 2.35] and [45.75±1.64] μm/s; VAP [18.40 ± 1.27] a

关 键 词：解脲脲原体 细胞色素氧化酶 知柏地黄汤 MRNA表达 

分 类 号：R285.5[医药卫生—中药学]