神经肽Y对癫痫大鼠海马AMPA受体GluR2亚单位功能的影响  

Effects of neuropeptide Y on GluR2 subunit in hippocampal AMPA receptor in epileptic rats

作　　者：任慧玲[1] 步玮[2] 赵庆林[2] 赵大巍[2] 

机构地区：[1]河北医科大学第三医院神经内科,石家庄050051 [2]河北医科大学第三医院神经外科,石家庄050051

出　　处：《中国临床药理学杂志》2017年第15期1447-1449,1453,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省引进留学人员基金资助项目(CY201609)

摘　　要：目的研究神经肽Y(NPY)对海人酸(KA)致癫痫大鼠AMPA受体GluR2亚单位功能的影响。方法用KA建立大鼠癫痫模型。将成年雄性SD大鼠随机分为对照组、模型组、实验A组、实验B组,每组10只。对照组予以0.9%Na Cl 1μL,于脑室注药点内注射,模型组予以5.5μg·μL^(-1)KA 1μL,于脑室注药点内注射;实验A组予以5μg·μL^(-1)NPY 1μL,于海马区注药点内注射;实验B组予以3μg·μL^(-1)BIBP3226 1μL,0.5 h后注入5μg·μL^(-1)NPY 1μL,于海马区注药点内注射。24 h后,实验A组和实验B组予以5.5μg·μL^(-1)KA 1μL,于脑室注药点内注射。用Western blot法检测4组大鼠海马CA3区的GluR2亚单位的总蛋白和磷酸化蛋白的变化,用实时荧光定量聚合酶链式反应(RT-PCR)法检测4组大鼠海马CA3区的GluR2 mRNA表达水平。结果对照组、模型组、实验A组和实验B组的GluR2亚单位的总蛋白分别为0.63±0.21,0.63±0.36,0.63±0.28和0.61±0.22,差异均无统计学意义(均P>0.05)。对照组、模型组、实验A组和实验B组的GluR2亚单位蛋白磷酸化分别为0.42±0.21,1.24±0.17,0.91±0.07和1.05±0.08,GluR2 mRNA分别为1.01±0.14,0.42±0.16,0.76±0.07和0.60±0.09,对照组与其他3组比较,差异均有统计学意义(均P<0.05),实验A组与模型组、实验B组比较,差异均有统计学意义(均P<0.05)。结论 NPY可以通过激活Y1受体,抑制大鼠癫痫发作,减轻癫痫所致的AMPA受体GluR2亚单位功能受抑制。Objective To investigate the effects of neuropeptide Y（ NPY） on GluR2 subunit in hippocampal AMPA receptor with epileptic rats by injecting kainic acid（ KA）. Methods The adult male SD rats with epileptic model were builded by KA. The rats were randomly divided into control,model,test A and test B groups. Control group was given 0. 9% Na Cl 1 μL,intraventricular injection. Model group was given5. 5 μg · μL^-1KA 1 μL,intraventricular injection. Test A group was given 5 μg · μL^-1NPY 1 μL,intraventricular injection. Test B group was given 3 μg·μL^-1BIBP3226 1 μL,then given 5 μg·μL^-1NPY 1μL after half hour,intraventricular injection. After 24 h,the test A and B groups received 5. 5 μg · μL^-1KA 1 μL,intraventricular injection.The total protein and phosphorylation protein of GluR2 in hippocampal CA3 were measured by Western blot,and the expression of GluR2 mRNAin hippocampal CA3 were measured by real-time fluorescent quantitative polymerase chain reaction（ RT-PCR）.Results The total protein of GluR2 in control,model,test A and test B groups were 0. 63 ± 0. 21,0. 63 ± 0. 36,0. 63 ± 0. 28 and 0. 61 ± 0. 22 without significant difference（ all P〈0. 05）. In control,model,test A and test B groups,the phosphorylation protein of GluR2 were 0. 42 ± 0. 21,1. 24 ± 0. 17,0. 91 ± 0. 07 and 1. 05 ± 0. 08,the GluR2 mRNA were 1. 01 ± 0. 14,0. 42 ± 0. 16,0. 76 ± 0. 07 and 0. 60 ± 0. 09. There were statistically significant differences between control group and other three groups（ all P〈0. 05）,and also between test A group and model,test B groups（ all P〈0. 05）. Conclusion NPY could inhibit the epileptic seizure of rats,which can reduce the AMPA receptor GluR2 subunit function were inhibited by epilepsy,with activating the Y1 receptor.

关 键 词：神经肽Y 癫痫 GluR2亚单位 

分 类 号：R971.6[医药卫生—药品]