正交设计法优化喜树碱胶束制备工艺的考察  

OPTIMIZATION OF PREPARING CAMPTOTHECIN MICELLES BYORTHOGONAL DESIGN

作　　者：刘佳[1] 贾娜[1] 吕晓洁[1] 李瑞娟[1] 赛那[1] 顾艳丽[1] 

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010010

出　　处：《内蒙古医科大学学报》2017年第3期244-248,共5页Journal of Inner Mongolia Medical University

基　　金：内蒙古自治区自然科学基金(2013MS1209)

摘　　要：目的:正交设计法优化喜树碱胶束的制备工艺。方法:采用透析法制备喜树碱胶束,在单因素考察的基础上,以投料比、p H值、透析温度为影响因素,药物包封率为指标,应用正交设计法优化载药胶束的制备工艺,并对最佳工艺进行重复性实验。结果:喜树碱胶束的最佳制备工艺为:聚合物材料与药物的投料比为1:10、p H值为5、透析温度为30℃。重复性实验结果表明,载药胶束的平均包封率为51.86%,RSD为0.11%。结论:最佳制备工艺制得胶束的包封率均高于正交试验结果,该制备工艺稳定可行,成功实现了难溶性药物喜树碱的增溶,为喜树碱胶束的制备提供了实验依据,为提高此类难溶性药物的临床疗效奠定了基础。Objective：The research aimed to optimize the preparation technology of camptothecin micelles by orthogonal design.Methods：The drug loaded micelles were prepared by dialysis method.The orthogonal tests were used to optimize the preparation technology involving feed ratio,pH value and temperature.Results：The optimal preparation technology of dialysis method was that the feeding ration was 1：10,pH value was 5,the temperature was 30℃.The repeat test shows that the encapsulation rate was up to51.86%with high stability as RSD=0.11%.Conclusion：The optimized preparation technology of camptothecin micelles was stable and feasible.Successfully solve the problem of insoluble drug CPT.

关 键 词：喜树碱 胶束 正交试验 

分 类 号：R94[医药卫生—药剂学]