氧化铜-过氧化聚吡咯-还原氧化石墨烯复合膜修饰电极非酶检测葡萄糖  

Non-enzymatic Detection of Glucose Using a Copper Oxide-Overoxidized Polypyrrole-Reduced Graphene Oxide Composite Film Modified Electrode

作　　者：于浩[1] 陈小霞[1] 冯晓[1] 王姗姗[1] 金君[1] YU Hao CHEN Xiao-xia FENG Xiao WANG Shan-shan JIN Jun(College of Chemistry and Chemical Engineering, Yan＇an University, Yan＇an Key Laboratory of Analytical Technology and Detection, Yan＇an 716000, China)

机构地区：[1]延安大学化学与化工学院延安市分析技术与检测重点实验室,陕西延安716000

出　　处：《分析测试学报》2017年第8期969-974,共6页Journal of Instrumental Analysis

基　　金：延安市科技局工业攻关项目(2014KG-01)

摘　　要：先以氧化石墨烯(Graphen oxide,GO)为阴离子掺杂剂,采用电化学聚合法制备了聚吡咯-氧化石墨烯复合膜(PPy-GO)。分别在0.10 mol/L Na Cl和0.10 mol/L NaOH溶液中对其进行还原和过氧化处理,制得过氧化聚吡咯-还原氧化石墨烯复合膜(OPPy-ERGO)。再以此OPPy-ERGO复合膜为载体,采用电化学沉积法制备了氧化铜-过氧化聚吡咯-还原氧化石墨烯复合膜修饰电极(CuO-OPPy-ERGO/CCE)。通过扫描电镜和电化学方法对此电极进行表征,研究了葡萄糖在此修饰电极上的电化学行为。结果表明,此电极对葡萄糖的电氧化过程表现出高的催化活性和良好的抗干扰能力。在0.20 mol/L NaOH溶液中,安培法检测葡萄糖的线性范围为5.0×10^(-7)~1.0×10^(-3)mol/L,检出限(3Sb)为2.0×10^(-7)mol/L,灵敏度为121.8μA/(mmol·L^(-1))。该电极用于血清中葡萄糖含量的测定,加标回收率为96.0%~110.1%。A copper oxide -overoxidized polypyrrole -reduced graphene oxide composite film（ CuO - OPPy - ERGO/CCE） modified electrode was fabricated by using layer by layer electrochemical depo- sition technique. Firstly, a polypyrrole （PPy） - graphene oxide （GO） composite film was prepared by electropolymerization of pyrrole while using GO as the anionic dopant. After the electrochemical reduction in 0. 10 mol/L NaCl solution and peroxidation in 0. 10 mol/L NaOH solution, an overoxi- zied polypyrrole - reduced graphene oxide （ OPPy - ERGO） composite film was obtained. Then, cop- per oxide was electrochemically deposited on this OPPy -ERGO film, and the modified electrode was obtained. The surface morphology and the electrochemical behavior of this modified electrode were in- vestigated by scanning electron microscopy（SEM） and cyclic voltammetry. Furthermore, the electro- chemical behavior of glucose on this electrode was also investigated carefully. The results showed that this modified electrode exhibited a high electrocatalytic activity and a good anti-interference ability for the oxidation of glucose. Under the optimum conditions, the linear range for the determination of glu- cose by amperommetry was in the range of 5.0 × 10-7 - 1.0 × 10-3 mol/L. The detection limit（3Sb =3） and the sensitivity was 2. 0 × 10-7 mol/L and 121.8 μA/（mmol · L-1）, respectively. The modified electrode was successfully used to determine glucose in human serum sample with recoveries of 96.0% - 110. 1%.

关 键 词：氧化铜 过氧化聚吡咯 还原氧化石墨烯 葡萄糖 电催化 

分 类 号：O657.1[理学—分析化学] TS245.4[理学—化学]