爆震伤对大鼠肺组织凋亡影响研究  

Effect of blast injury on apoptosis of lung tissue in rats

作　　者：丛培芳[1] 柳云恩[1] 张玉彪[1] 佟昌慈 史秀云[1] 刘颖[1] 施琳[1] 佟周[1] 金红旭[1] 侯明晓[1] 

机构地区：[1]沈阳军区总医院急诊医学部全军重症(战)创伤救治中心实验室辽宁省重症创伤和器官保护重点实验室,辽宁沈阳110016

出　　处：《创伤与急危重病医学》2017年第4期205-209,214,共6页Trauma and Critical Care Medicine

基　　金：全军十二五面上项目(CSY12J002);全军十二五面上项目(CSY13J002);全军重大新药创制项目(2013ZX09J13109-02B);总后卫生部重大新上(ASM14L008)

摘　　要：目的通过建立肺爆震伤大鼠模型,检测不同时间点大鼠肺组织中c-Jun氨基末端激酶(JNK)、P38、Bad及Bcl-xl的变化,探讨JNK/P38通路在肺爆震伤中的作用,旨在为肺爆震伤的损伤机制提供理论依据。方法选取18只成年健康雄性SD大鼠,分别纳入对照组与爆震伤后24 h组、7 d组,每组各6只。TUNEL检测爆震伤后肺组织的凋亡情况;免疫荧光检测JNK与P38的表达情况;Western-blot检测JNK、P38、Bcl-xl与Bad的蛋白表达情况;Real Time PCR检测JNK、P38、Bcl-xl与Bad的m RNA表达情况。结果 TUNEL结果显示,与对照组比较,爆震伤后24 h组的肺组织细胞凋亡率明显升高;与24 h组比较,7 d组凋亡率降低,差异均有统计学意义(P<0.05)。免疫荧光结果显示,与对照组比较,JNK与P38在爆震伤后24 h表达升高;与24 h组比较,7 d组表达降低,差异均有统计学意义(P<0.05)。Western-blot与Real Time PCR结果显示,与对照组比较,JNK、P38及Bad在爆震伤后24 h蛋白与m RNA表达均升高;与24 h组比较,7 d组表达均降低,差异有统计学意义(P<0.05)。而Bcl-xl在爆震伤后24 h蛋白与m RNA表达低于对照组,7 d组表达高于24 h组,差异有统计学意义(P<0.05)。结论肺爆震伤后的细胞凋亡反应受JNK/P38通路的调控,且与时间具有相关性。Objective Through establishment of rats model with blast injury in lungs, to detect the changes of different time points in lung tissue of c-Jun amino terminal kinase （JNK）, P38, Bad and Bcl-xl, to investigate the role of JNK/P38 pathway in blast injury in lungs,and to provide the theory basis for damage mechanism. Methods A total of 18 adult healthy female SD rats were selected and divided into the control group, the group of 24 hours after blast injury （group of 24 hours）and the group of 7 days after blast injury （group of 7 days）, with 6 cases in each group. Apoptosis in lung was observed by TUNEL stain. Immunofluorescence was used to detected the expression of JNK and P38. The expressions of JNK, P38, Bcl-xl and Bad were detected by Western-blot. The expression of mRNA in these factors such as JNK, P38, Bcl-xl and Bad were detec- ted by Real Time PCR. Results The result of TUNEL showed that, compared with the control group,the apoptosis rate of lung tissue in the group of 24 hours was significantly increased;compared with the group of 24 hours, the apoptosis rate in the group of 7 days was reduced, and the difference had statistical significance （ P 〈 0. 05 ）. The result of immunofluores- cence showed that, compared with the control group, the expression of JNK and P38 increased after 24 hours of blast injury; compared with the group of 24 hours, the expression in 7 days reduced （ P 〈 0. 05 ）. The results of Western-blot and Real Time PCR showed that, compared with the control group, the expression of mRNA in JNK, P38 and Bad after 24 hours of in- jury were increased ; compared with the group of 24 hours, the expression in 7 days reduced （ P 〈 0. 05）. The expression of Bcl-xl in the 24 hours protein and mRNA was lower than that in the control group after the blast injury, and the expression in 7 days was higher than that in the group of 24 hours, and the difference was statistically significant （ P 〈 0. 05 ）. Conclu- sion The cell apoptosis after lung burst was regulated by

关 键 词：爆震伤 大鼠 C-JUN氨基末端激酶 P38 

分 类 号：R135.2[医药卫生—劳动卫生]