白屈菜总碱对舞毒蛾离体酶活性的影响  

Effects of Total Alkaloids from the Chelidonium majus on the Enzymes Activities in Lymantria dispar in Vitro

作　　者：吕春鹤[1] 张国财[1] 邹传山[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《中国林副特产》2017年第4期33-36,38,共5页Forest By-product and Speciality in China

摘　　要：通过对白屈菜粗提物纯化获得白屈菜总碱,对舞毒蛾3龄幼虫5种离体酶活进行测定。测定结果表明:用AB-8大孔吸附树脂纯化白屈菜粗提物,得到5种生物单碱,含量从高到低依次为:黄连碱>白屈菜碱>盐酸小檗碱>血根碱>四氢黄连碱。纯化后的白屈菜总碱对5种离体酶活均有不用程度的抑制作用,对GST抑制作用最强,IC_(50)为5.333mg/mL,对CarE的抑制作用最弱,IC_(50)为3.475×10~3 mg/mL。白屈菜总碱对舞毒蛾3龄幼虫5种离体酶的敏感性依次为GST>AChE>CAT>SOD>CarE。Purify the crude extract of Chelidonium majus to get the total alkaloids of Chelidonium,determine the five in vitro enzyme activities of 3rd instar larvae. The results show： five main isoquinoline alkaloids in C. majus are suc- cessfully isolated using macroporous resin AB-8 adsorption, content from high to low followed by coptisine〉cel- eryridine 〉berberine hydrochloride 〉 sanguinarine 〉 tetrahydrocoptisine, the strongest inhibitory activity to glu- tathione S-transferase （GST） IC50 ;5. 333mg/mL, and the weakest inhibition to carboxylesterase （CarE）. ICs0：3. 475 X 103 ml〉mL. The IC50 values of five enzymes is in a decreasing order of GST〉AChE〉CAT〉SOD〉CarE.

关 键 词：白屈菜 舞毒蛾 生物碱 离体酶活 

分 类 号：S767.3[农业科学—森林保护学]