Examine the Correlation between Heat Shock Protein IbpA and Heat Tolerance in Cronobacter sakazakii  

作　　者：ZHAO Zhi Jing WANG Bin YUAN Jing LIANG Hao Yu DONG Si Guo ZENG Ming 

机构地区：[1]National Institutes for Food and Drug Control, Beijing 100050, China [2]Institute of Disease Control and Prevention,Academy of Military Medical Sciences, Beijing 100850, China

出　　处：《Biomedical and Environmental Sciences》2017年第8期606-610,共5页生物医学与环境科学（英文版）

基　　金：funded by National Science and Technology Major Project of the Ministry of Science and Technology of China(2013ZX09304101)

摘　　要：We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 AibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 AibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coil O157：H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 AibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 AibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coil O157：H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.

关 键 词：Examine the Correlation between Heat Shock Protein Ibp A and Heat Tolerance in Cronobacter sakazakii HEAT CMCC 

分 类 号：R446.5[医药卫生—诊断学]