白藜芦醇抑制小肠缺血再灌注继发肺损伤中P38MAPK通路及下游凋亡、炎症、氧化应激分子的表达  

Resveratrol inhibits the P38MAPK pathway as well as downstream apoptosis,inflammation and oxidative stress molecule expression in secondary lung injury after intestinal ischemiareperfusion

作　　者：王晓光[1] 齐晓非[1] 李戈辉[1] WANG Xiao-guang QI Xiao-fei LI Ge-hui(Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518028)

机构地区：[1]深圳市妇幼保健院麻醉科,广东深圳518028

出　　处：《海南医学院学报》2017年第12期1603-1606,共4页Journal of Hainan Medical University

基　　金：广东省卫生厅科研项目(JCYJ20160427192121979)~~

摘　　要：目的:研究白藜芦醇对小肠缺血再灌注继发性肺损伤的保护作用及其对P38MAPK通路功能的影响。方法:选择成年雄性SPF级SD大鼠并分为对照组、I/R组、Res-L组、Res-M组、Res-H组,建立小肠缺血再灌注模型,Res-L组、Res-M组、Res-H组分别给予5.0mg/kg、10.0mg/kg、15.0mg/kg白藜芦醇干预。检测肺脏中P38MAPK通路分子及下游凋亡分子、炎症因子、氧化应激产物的含量。结果:I/R组大鼠肺组织中P38MAPK、MAPKK、MAPKKK、Fas、FasL、caspase-8、caspase-9、NF-kB、TNF-α、IL-1β的表达量以及显ROS、MDA的含量显著高于对照组;Res-L组、Res-M组、Res-H组大鼠肺组织中P38MAPK、MAPKK、MAPKKK、Fas、FasL、caspase-8、caspase-9、NF-kB、TNF-α、IL-1β的表达量以及显ROS、MDA的含量著低于I/R组。结论:白藜芦醇能够通过抑制P38MAPK通路功能来减轻细胞凋亡、炎症反应和氧化应激反应,进而保护小肠缺血再灌注继发性肺损伤。Objective：To study the protective effect of resveratrol on secondary lung injury induced by intestinal ischemia reperfusion and its effect on P38 MAPK pathway.Methods：Adult male SPF SD rats were divided into control group,I/R group,Res-L group,Res-M group,Res-H group,the small intestine ischemia reperfusion model was made and Res-L group,Res-M group,Res-H group were given 5.0mg/kg,10.0mg/kg,15.0 mg/kg resveratrol.P38 MAPK pathway molecules and downstream apoptotic molecules,inflammatory factors and oxidative stress products in the lung were determined.Results：P38MAPK,MAPKK,MAPKKK,Fas,FasL,Caspase-8,caspase-9,NF-kB,TNF-α,IL-1βexpression and ROS,MDA contents in lung tissue of I/R group were significantly higher than those of the control group;P38MAPK,MAPKK,MAPKKK,Fas,FasL,Caspase-8,caspase-9,NF-kB,TNF-α,IL-1βexpression and ROS,MDA contents in lung tissue of Res-L group,Res-M group and Res-H group were significantly lower than those of I/R group.Conclusions：resveratrol can inhibit apoptosis,inflammation and oxidative stress by inhibiting the function of P38 MAPK pathway,and then protect the secondary lung injury induced by intestinal ischemia reperfusion.

关 键 词：白藜芦醇 缺血再灌注 肺损伤 P38MAPK 

分 类 号：R285.5[医药卫生—中药学]