电针对坐骨神经损伤大鼠BDNF、NGF和GAP-43表达的影响  

Effect of Electroacupuncture on Expression of Brain-derived Neurotrophic Factor,Nerve Growth Factor,and Growth-associated Protein-43 in Rats with Sciatic Nerve Injury

作　　者：叶伊琳[1] 唐洁[1] 李莉[2] YE Yi-lin TANG Jie LI Li(School of Nursing, Anhui University of Chinese Medicine, Anhui Hefei 230012, China Clinical School of Integrated Traditional Chinese and Western Medicine ,Anhui University of Chinese Medicine, Anhui Hefei 230038,China)

机构地区：[1]安徽中医药大学护理学院,安徽合肥230012 [2]安徽中医药大学中西医结合学院,安徽合肥230038

出　　处：《安徽中医药大学学报》2017年第4期51-55,共5页Journal of Anhui University of Chinese Medicine

基　　金：安徽高校省级自然科学基金项目(KJ2015A116)

摘　　要：目的观察电针对大鼠坐骨神经损伤的保护作用,探究其作用机制。方法取雄性SD大鼠,随机分成假手术组、模型组、电针组。除假手术组外,其他各组采用钳夹法复制坐骨神经损伤模型,2d后对电针组大鼠"足三里"和"环跳"进行电针治疗,7d为1个疗程,共治疗2个疗程,并分别在7d和14d对大鼠坐骨神经功能进行评价,治疗结束后,采用免疫组织化学法对坐骨神经中脑源性神经营养因子(brain derived nerve growth factor,BDNF)、神经生长因子(nerve growth factor,NGF)和生长相关蛋白-43(growth associated protein-43,GAP-43)进行检测,同时在光镜下观察坐骨神经的病理变化。结果与模型组比较,电针组大鼠坐骨神经功能指数(sciatic nerve function index,SFI)明显增加,最大诱发电位和神经传导速度(nerve conduction velocity,NCV)明显加快,潜伏期明显缩短。病理检查显示电针组神经纤维排列相对整齐,空泡样变性减少,可见到施万细胞的增殖。坐骨神经中BDNF、NGF和GAP-43表达明显上调。结论电针治疗能促进大鼠坐骨神经损伤修复,可能与其上调BDNF、NGF和GAP-43表达有关。Objective To investigate the protective effect of electroacupuncture in rats with sciatic nerve injury and possible mechanisms.Methods Male Sprague-Dawley rats were randomly divided into sham-operation group, model group, and electroacupuncture group.The rats in the model group and electroacupuncture group were treated with clamp technique to establish a rat model of sciatic nerve injury.Two days later, the rats in the electroacupuncture group were given electroacupuncture at ＂Zusanli＂ and ＂Huantiao＂ points for 2 courses of treatment （7 days per course of treatment）, and the function of the sciatic nerve was assessed on days 7 and 14 of treatment.After the treatment ended, immunohistochemistry was performed to measure the expression of brain-derived neurotrophic factor （BDNF）, nerve growth factor （NGF）, and growth-associated protein-43 （GAP-43） in the sciatic nerve, the pathological changes of the sciatic nerve were observed under a light microscope.Results Compared with the model group, the electroacupuncture group had significant increases in sciatic nerve function index, maximum evoked potential, and nerve conduction velocity and a significant reduction in latency period.Pathological examination showed well arranged nerve fibers, reduced vacuolar degeneration, proliferation of Schwann cells, and significantly upregulated expression of BDNF, NGF, and GAP-43 in the electroacupuncture group.Conclusion Electroacupuncture may promote the repair of sciatic nerve injury in rats, possibly by upregulating the expression of BDNF, NGF, and GAp-43.

关 键 词：电针 坐骨神经 脑源性神经营养因子 神经生长因子 生长相关蛋白 

分 类 号：R245.32[医药卫生—针灸推拿学]