ApoE基因多态性与血脂、氧化低密度脂蛋白、糖尿病足的相关性分析  

Relativity among ApoE Gene Polymorphism and Lipid,Oxidized Low-density Lipoprotein and Diabetic Foot

作　　者：叶炳华[1] 孙筱晔 蒋泽[1] 戚文群[1] 唐宝康[1] 倪云[1] YE Bing-hua SUN Xiao-ye JIANG Ze et al(Taizhou Third People＇s Hospital, Taizhou 225321, China)

机构地区：[1]江苏省泰州市第三人民医院,江苏泰州225321

出　　处：《中国医学创新》2017年第23期49-52,共4页Medical Innovation of China

基　　金：江苏省泰州市社会发展计划项目(201216)

摘　　要：目的:评价载脂蛋白E(ApoE)基因多态性与血脂、氧化低密度脂蛋白(oxLDL)及2型糖尿病(T2DM)患者发生糖尿病足(DF)的相关性,为DF的早期基因诊断提供循证医学的证据。方法应用聚合酶链反应-限制性片段长度多态性检测70例T2DM患者(40例DF患者,30例无慢性并发症患者)和48例健康对照者的ApoE基因多态性,比较T2DM患者中并发DF(DF组)与无慢性并发症DM(DM对照组)患者及健康组的Apo E基因型分布和等位基因频率,并利用Logisitic回归分析ApoE基因多态性与DF之间的相关性。结果:DF组ε3/4基因型和ε4等位基因均高于其他两组;ε2/4、ε3/4两组TCH、LDL、oxLDL、HbA1C均明显高于ε2/3、ε3/3两组,HDL则低于上述两组;各等位基因之间比较:ε4组TCH、LDL、oxLDL、HbA1C均显著高于ε2、ε3两组,ε2组TG明显高于ε3、ε4两组;ε3/4基因型和ε4等位基因与发生DF明显相关。结论 ApoE基因多态性与T2DM患者DF的发生相关,基因型ε3/4和等位基因ε4可能是T2DM患者并发DF的危险因子,其机制可能通过血脂代谢紊乱及促进脂质氧化而影响DF的发生。Objective： To investigate the association of apolipoprotein E （ ApoE ） gene polymorphism with diabetic foot （ DF ） in patients with type 2 diabetes mellitus （ T2DM ） and hyperlipidemia （ LDL ）, and to provide the evidence of evidence-based medicine for early gene diagnosis of DF.Method： Firstly, polymorphism of ApoE gene was detected by polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP ） in 70 T2DM patients （ 40 with DF while 30 without chronic complications ） and 48 healthy controls.Secondly, ApoE genotype distribution and allele frequency of DF （ DF group ） and non-chronic complications DM （ DM control group ） patients and health group control group were investigated.Correlation between ApoE gene polymorphism and DF was also analyzed with logistic regression analysis.Result： ε 3/4 genotype and ε 4 allele in DF group were significantly higher than those in the other two groups （ P〈0.05 ） .Besides HDL, the levels of TCH, LDL, oxLDL and HbAlc in ε 2/4 and ε 3/4 were significantly higher than those in ε 2/3 and ε 3/3 （P〈0.05） .The levels of TCH, LDL, oxLDL and HbAlc in ε 4 were significantly higher than those in ε2 and ε3, while the level of TG in ε 2 was significantly higher than that in ε 3 and ε 4 （ P〈0.05 ） . ε 3/4 genotype and ε 4 allele were significantly associated with DF.Conclusion： ApoE gene polymorphism is associated with the occurrence of DF in T2DM patients.Genotype ε 3/4 and allele ε 4 are risk factors probably for DF in T2DM patients.The mechanism may be related to lipid metabolism disorder and lipid oxidation.

关 键 词：载脂蛋白E 糖尿病足 血脂 氧化低密度脂蛋白 2型糖尿病 

分 类 号：R587.2[医药卫生—内分泌]