小鼠CD4T细胞转化的双阴性T细胞的效应分子研究  

Effector molecules of mouse CD4 T cell converted double negative T

作　　者：田丹[1] 孙广永[1] 刘锴[1] 田月[1] 施文[1] 王天琪[1] 金华[1] 张春盼 张栋[1] cell Tian Dan Sun Guangyong Liu Kai Tian Yue Shi Wen Wang Tianqi JiM Hua Zhang Chunpan Zhang Dong .(Experimental and Translational Research Center, Beifing Friendship Hospital, Capital Medical University, Beijing 100050, China)

机构地区：[1]首都医科大学附属北京友谊医院科研实验中心,北京100050

出　　处：《国际外科学杂志》2017年第7期459-463,F0003,共6页International Journal of Surgery

基　　金：北京市自然科学基金（7172060）;国家自然科学基金（81601388）

摘　　要：目的研究小鼠CD4T细胞转化的CD4-CD8双阴性T细胞的功能特点，进一步明确双阴性T细胞的免疫抑制作用机制。方法应用转录组测序和蛋白质质谱技术分析了小鼠转化的双阴性T细胞基因表达谱。应用流式细胞术等技术验证了细胞活化分子CD44、CD69和OX40的表达水平并应用CFSE染色法验证双阴性T细胞的杀伤功能。结果小鼠转化的双阴性T细胞表达了与其他成熟CD4T细胞不同的细胞表型。小鼠转化的双阴性T细胞明显高表达细胞表面活化分子CD44、CD69和OX40，以及颗粒酶、穿孔素等细胞杀伤相关基因。穿孔素基因敲除的双阴性T细胞抑制淋巴细胞增殖的能力明显降低。结论小鼠CIMT细胞转化的双阴性T细胞是与其他成熟CD4T细胞不同的T细胞终末分化亚群。小鼠转化的双阴性T细胞高表达细胞活化分子和免疫杀伤类细胞因子，并主要通过穿孔素途径发挥免疫抑制功能。Objective To investigate the function characteristics of CD4 T cell converted double negative T cell and provide a basis for further insight into the characteristics of mouse converted double negative T cell. Methods The gene expression profile was analyzed by transcriptome sequencing and protein mass spectrometry. The expression of cell active marker CD44, CD69 and OX40 was investigated by flow cytometry and the cytotoxicity of mouse double negative T cell was verified by CFSE staining. Results Mouse CD4 T cell converted double negative T cell expressed cell phenotype that differed from other mature CD4 T cells. Mouse converted double negative T cell expressed high level of active marker of CD44, CD69 and OX40. Cytdtoxicity of PrJ~~ DN T was significantly reduced. Conclusions Mouse CD4 T cell converted double negative T cell has distinguishing cell phenotypes, that are not identical to other mature CD4 T cells. Mouse double negative T cell overexpresses cell activation marker and cytotoxic cytokines. The immune suppressive function of mouse double negative T cell is mainly dependent on perforin pathway.

关 键 词：T淋巴细胞 双阴性T细胞 细胞转化 肿瘤 转录组测序 

分 类 号：R392.4[医药卫生—免疫学]