乙肝病毒基因分型悬液芯片检测方法建立及初步应用  

Establishment and application of a multiplex liquid bead array for typing of hepatitis B virus

作　　者：李云[1] 史玲莉[1] 闫冀焕[1] 沈军[1] 李薇[1] 滑娜[2] 

机构地区：[1]河北国际旅行卫生保健中心,河北石家庄050000 [2]河北出入境检验检疫局曹妃甸办事处

出　　处：《中国国境卫生检疫杂志》2017年第3期159-163,共5页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的建立基于悬液芯片的乙肝病毒(HBV)基因型检测方法。方法根据现有HBV 8个基因型的DNA序列信息,设计并合成相关引物及探针,首先进行PCR扩增,产物与核酸探针微球组杂交后检测荧光信号值。结果建立的悬浮芯片方法检测HBV-A、HBV-E、HBV-G的敏感性为9DNA拷贝,HBV-B、HBV-C、HBV-D、HBV-F、HBV-H的敏感性为90DNA拷贝。检测结果与荧光PCR方法相比,差异无统计学意义。结论建立了可同时检测8个HBV基因型的悬液芯片检测方法,为快速筛查和鉴定HBV提供了新的手段。Objective To establish a method for rapid detecting and genotyping of eight genotypes of hepatitis B virus（HBV） based on liquid bead array. Methods Primers and probes were designed and synthesized according to genomic sequences in Gen Bank. Viral DNA was amplified by using multiplex PCR. The PCR products were hybridized with beads coupled with nucleic acid probe,and the fluorescence signals were detected by Bio-Plex 200 system.Results The limitation of detection for HBV-A、HBV-E、HBV-G were about 9DNA copies,and about 90 DNA copies for HBV-B、HBV-C、HBV-D、HBV-F、HBV-H. There was no significant difference between the liquid bead array and fluorescent PCR kit. Conclusion A liquid bead array assay of simultaneous detection for eight genotypes of HBV was developed,which will provide a new method for rapid screening and identification of HBV.

关 键 词：乙肝病毒 悬液芯片 基因 型别 

分 类 号：R446.119[医药卫生—诊断学]