东乡野生稻芒长的QTL定位  

Mapping QTLs Controlling Awn Length in Dongxiang Wild Rice

作　　者：郑剑[1] 周军杰[1] 李兴星[1] 苏华英[1] 南文斌 秦小健[1] 杨永清[1] 张汉马 梁永书[1] Zheng Jian Zhou Junjie Li Xingxing Su Huaying Nan Wenbin Qin Xiaojian Yang Yongqing Zhang Hanma Liang Yongshu(Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing, 401331)

机构地区：[1]重庆师范大学植物环境适应分子生物学重庆市重点实验室,重庆401331

出　　处：《分子植物育种》2017年第7期2678-2686,共9页Molecular Plant Breeding

基　　金：国家973前期专项(2014CB160306);水稻生物学国家重点实验室开放课题(150201);重庆市自然科学基金(Cstc2014jcyjA 80003);重庆市教委项目(KJ1400516);重庆师范大学博士启动基金(13XLB017)共同资助

摘　　要：为了揭示东乡野生稻芒长的遗传规律。本研究以"东乡野生稻/93-11"F_1、F_2和F_(2:3)群体为遗传材料,构建了1张含163个SSR标记的遗传连锁图谱。考查2群体各株系芒长性状值,利用SPSS 19.0、Mapmaker/Exp 3.0和WinQTLCart 2.50软件进行数据统计分析、遗传图谱绘制和QTL定位。结果表明,有芒对无芒在F_1植株呈显性遗传,稻芒的长短在F_2和F_(2:3)群体中呈连续正态分布,表现为数量性状遗传。在F_2和F_(2:3)群体中共检测到7个芒长QTLs,分布在第3、第4、第5、第8、第10和第11号染色体上,贡献率介于1.93%~56.68%。其中,第4号染色体RM5687标记附近两群体均检测到1个芒长QTL(qAL4a和qAL4b)。在F_2和F_(2:3)群体中共检测到3对芒长上位性QTLs。芒长以加/显/上位性效应遗传模式共同作用,遗传基础较复杂。本研究所检测到的QTL为揭示东乡野生稻芒长进化的遗传机理奠定基础。To elucidate the genetic mechanism of awn length in Dongxiang wild rice（DXWR）, the genetic linkage map was constructed with the F2 population derived from DXWR×93-11 and containing 163 SSR markers using Mapmaker/Exp 3.0 software. Phenotypic values of awn length in the F1, F2 and F2：3 populations were investigated for QTL mapping using Win QTLCart 2.50 and SPSS 19.0 software, respectively. The results indicated that the presence or absence of awn showed dominant genetic in the F1 plant, phenotypic value of awn length showed continuous distribution and controlled by quantitative trait locus（QTL） in the F2 and F2：3 populations. Altogether,there 7 QTLs were detected for awn length and distributed on chromosomes 3, 4, 5, 8, 10 and 11 in the two populations, phenotypic variance explained by single QTL from 1.93% to 56.68%. Among of them, q AL4 a and q AL4 b for awn length were detected repeatedly RM5687 on chromosome 4 in the both F2 and F2：3 populations.Three pairs of epistasis QTLs were detected for awn length in the F2 and F2：3 populations. The genetic model of awn length was cooperated by additive-dominance-epistasis effect in the two populations. This will provide valuable insights into the molecular mechanism underlying awn length of DXWR.

关 键 词：东乡野生稻 芒长 分子标记 遗传分析 QTL 

分 类 号：Q943.2[生物学—植物学] S511.9[农业科学—作物学]