姜荷花‘玉如意’种球的抗寒性研究  

Study on Cold Resistance of Curcuma hybrid 'Laddawan' bulbs

作　　者：刘建新[1] 徐笑寒[1] 丁华侨[1] Liu Jianxin Xu Xiaohan Ding Huaqiao(Research ＆ Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Hangzhou, 311202)

机构地区：[1]浙江省农业科学院花卉研究开发中心,杭州311202

出　　处：《分子植物育种》2017年第7期2796-2803,共8页Molecular Plant Breeding

基　　金：杭州市科技计划引导项目(20163501Y78);浙江省农业科学院的公共发展专项;青年人才培养项目;科技产业化资金项目共同资助

摘　　要：姜荷花是国内新兴的一种热带花卉,但中国绝大部分地区冬季寒冷导致种球不能自然越冬保存。为了明确姜荷花‘玉如意’种球的抗寒生理、并找出提升种球抗寒性的方法。本研究对经不同低温胁迫处理‘玉如意’种球的各种生理生化指标进行了检测,并开展了种球的抗寒药剂筛选试验。种球抗寒性生理生化指标检测试验表明,‘玉如意’种球在9℃时开始受逆境胁迫;根据抗氧化酶系统和渗透调节系统的变化可知,在5℃和1℃时种球调动了自身的抗逆系统抵御环境的不良影响;而在-3℃时,各抗逆相关指标基本上降低到最低点,超出了种球的耐受极限。抗寒药剂筛选试验表明,各药剂处理(包括脱落酸(ABA),水杨酸(SC),芸苔素内酯(BR),6-BA,多效唑(PP333)等)对增强种球的抗寒性都有一定的作用。根据细胞受损程度分析,ABA、6-BA和PP333处理对种球的保护效果较好。ABA主要通过调整渗透调节系统来增加种球抗寒性,而6-BA则主要是通过调节抗氧化酶系统来达到目的。SC和BR处理的抗寒效果稍差。Curcuma alismatifolia is a new kind of tropical flowers. However, winter of most areas in China is too cold to preserve bulbs naturally. In order to clarify cold-resistance physiology of Curcuma hybrid ＇Laddawan＇（A variety of Curcuma alismatifolia） bulbs, and to find out methods to enhance bulbs cold-resistance, we have detected various physiological and biochemical indexes of bulbs treated with different low temperature stress, and carried out cold-resistant chemicals screening experiment of bulbs. The results of physiological and biochemical tests showed that Curcuma hybrid ＇Laddawan＇ bulbs began to be stressed at 9℃. According to changes of antioxidant enzyme system and osmotic adjustment system, these bulbs mobilized their own stress-resistant system to resist adverse effects of low temperature at 5℃ and 1℃; While at-3℃, various stress-resistant related indexes reduced to the lowest point, which beyond the tolerance limit of bulbs. The cold-resistant chemicals screening experiment showed that all chemicals treatments（Including abscisic acid（ABA）, salicylic acid（SC）, brassinolide（BR）, 6-BA,paclobutrazol（PP333） can enhance bulbs cold tolerance. According to cell damage degree analysis, ABA, 6-BA and PP333 treatment has fine protective effect to bulbs. ABA increase bulb cold resistance mainly through the adjustment of osmotic adjustment system, while 6-BA treatment mainly through the regulation of antioxidant enzyme system to achieve the purpose. cold resistance of SC and BR treatment were a bit poor.

关 键 词：姜荷花 玉如意 种球 抗寒性 脱落酸 

分 类 号：S682.29[农业科学—观赏园艺]