内皮细胞Glycocalyx成分在尿毒症患者及健康人血清中的差异表达  

The serum expression of the endothelial glycocalyx in uremic patients and healthy individuals

作　　者：孔祥雷[1] 韩雯雯[2] 苏红[1] 王尊松[1] 唐利军[1] 李文斌[1] 崔美玉[1] 许冬梅[1] Kong Xianglei Han Wenwen Su Hong et al(Department of Nephrology, Qianfoshan Hospital Mfiliated to Shandong University, Jinan250014, China Medical college of Shandong University, Jinan250014, China)

机构地区：[1]山东大学附属千佛山医院肾内科,山东济南250014 [2]山东大学医学院,山东济南250014

出　　处：《国际泌尿系统杂志》2017年第4期563-566,共4页International Journal of Urology and Nephrology

摘　　要：目的 探讨尿毒症患者血管内皮细胞糖蛋白复合物Glycocalyx成分在血清中的表达情况.方法 以23例未透析的尿毒症患者为研究对象,选取年龄及性别相匹配的15例健康志愿者作对照,收集其临床资料（如年龄、性别、高血压、糖尿病病史等）,应用自动生化分析仪检测血红蛋白、血白蛋白、血肌酐、血糖、血脂等,测定C反应蛋白,应用ELLISA法测定内皮细胞活化的细胞因子E选择素（ES）,同时测定血清Glycocalyx中的成分透明质酸（HA）和Syndecan-1（SDC）的水平.结果 该23例尿毒症患者平均年龄为（50.7 ± 11.8）岁,男性占52.2%.尿毒症患者血清中ES、HA、SDC的表达明显高于健康组,分别为（12.76±3.17）与（6.49±1.42） ng/mL,（5.94±2.91）与（2.33±0.73） ng/mL和535.9（363.0-935.7）与219.8（75.2-233.2） ng/mL,P〈0.05.Pearson相关分析显示,SDC与HA正相关（r=0.55,P〈0.05）,SDC与ES负相关（r=-0.43, P〈0.05）,而HA与ES不相关（r=-0.25,P〉0.05）.结论 尿毒症患者血清中内皮细胞糖蛋白复合物Glycocalyx成分表达升高,这可能与内皮细胞的激活有关.Objectives To explore the serum expression of the endothelial glycocalyx in uremic patients and healthy individuals.Methods Twenty-three uremic patients who not received dialysis and fifteen age-and sex-matched healthy individuals were included in the study.Sociodemographic characteristics such as age, sex, hypertension and diabetes were obtained by means of questionnaire.Blood haemoglobin, serum albumin, serum creatinine, blood glucose, serum total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides were also measured by automatic biochemistry analyzer.C-reactive protein was measured by immune transmission turbidimetry assay.E-selectin （ES）, hyaluronic acid （HA） and Syndecan-1 （SDC） were determined by indirect sandwich ELLISA methods.Results The uremic patients comprised 52.2% men, and the age was （50.7 ± 11.8）years.The serum levels of ES, HA and SDC were statistically higher in the uremic patients when compared with healthy controls, which was（12.76±3.17）vs.（6.49±1.42） ng/mL （P〈0.05）, （5.94±2.91） vs.（2.33 ± 0.73）ng/mL （P〈0.05） and 535.9 （363.0-935.7） vs.219.8 （75.2-233.2）ng/mL （P〈0.05）,respectively.Pearson＇s correlation revealed that SDC was significantly correlated with HA（r=0.55,P〈0.05）and ES（r=-0.43, P〈0.05）, but HA was not correlated with ES（r=-0.25, P〉0.05）.Conclusions The serum levels of endothelial glycocalyx are expressed higher in uremic patients than healthy controls, which will be related to the activation of vascular endothelial cells.

关 键 词：尿毒症 内皮细胞 糖蛋白类 

分 类 号：R692.5[医药卫生—泌尿科学]