创伤性休克致兔急性肺损伤中JAK／STAT通路的活化情况  

Molecular expression of JAK/STAT pathway in acute lung injury induced by traumatic shock

作　　者：史秀云[1] 柳云恩[1] 张玉彪[1] 佟昌慈 刘学磊[1] 刘颖[1] 丛培芳[1] 施琳[1] 金红旭[1] 高燕[1] 侯明晓[1] 

机构地区：[1]沈阳军区总医院急诊医学部全军重症(战)创伤救治中心实验室辽宁省重症创伤和器官保护重点实验室,辽宁沈阳110016

出　　处：《中国急救医学》2017年第8期754-757,共4页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨创伤性休克致兔急性肺损伤（ALI）中Janus激酶／信号转导与转录激活子（JAK／STAT）通路在不同时间点的表达变化情况。方法30只实验兔随机分为对照组、0h模型组、6h模型组、12h模型组和24h模型组，分别取得各组动物的血浆样本和肺组织样本，通过ELISA、Western blot以及实时荧光定量PCR等技术检测不同时间点髓过氧化物酶（MPO）、CC16、JAK和STAT的表达。结果MPO在模型组液体复苏后有所升高，但均低于对照组（P〈0．05），模型组肺组织CC16在0h表达明显升高，6h表达下降，12h表达继续下降，24h表达有所升高，差异具有统计学意义（P〈0．05）。同时模型组JAK2和STAT3表达也明显升高，模型组6h和12h表达下降（P〈0．05），24hSTAT表达有所下降，但与对照组比较差异无统计学意义（P〉0．01）。结论创伤性休克致兔ALI中JAK／STAT通路的相关蛋白表达被活化。Objective To investigate the expression changes of JAK/STAT pathway in the traumatic shock induced acute lung injury in rabbits. Methods Thirty experimental rabbits were randomly divided into control group, 0 h model group, 6 h model group, 12 h model group and 24 h model group. The serum and tissues were immediately removed from rabbits after molding. ELISA, Western blot and real - time PCR techniques were conducted to measure the expression of CC16, JAK and STAT at different time points. Result Myeloperoxidase（MPO） increased after fluid resuscitation in model group, which was lower than that in control group （P 〈0.05）. The expression of CC16 in lung tissue increased significantly at 0 h, reduced at 6 h and 12 h and then slightly increased at 24 h （ P 〈 0.05）. JAK2 and STAT3 expression also increased in model group and decreased at 6 h and 12 h （P 〈 O. 05 ）. The expression of STAT3 reduced slightly at 24 h, but there was no statistically significant difference compared with that in control group （P 〉 0.01 ）. Conclusion Proteins related to JAK/STAT pathway are activated in traumatic shock- induced acute lung injury in rabbits.

关 键 词：创伤性休克 急性肺损伤(ALI) Janus激酶/信号转导与转录激活子(JAK/STAT)通路 兔 

分 类 号：R631[医药卫生—外科学]