褐飞虱成虫唾液细菌蛋白的鉴定  

Identification of bacterial proteins in saliva of adults of the brown planthopper,Nilaparvata lugens(Hemiptera:Delphacidae)

作　　者：苗雨桐 邓瑶[1] 刘玉娣[1] 侯茂林[1] MIAO Yu-Tong DENG Yao LIU Yu-Di HOU Mao-Lin(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2017年第7期772-781,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31370439)

摘　　要：【目的】通过鉴定褐飞虱Nilaparvata lugens(St?l)水状唾液中的细菌蛋白,了解其唾液中的细菌种类。【方法】利用双层膜夹蔗糖溶液的方法对褐飞虱成虫唾液进行收集,超滤浓缩后进行电泳,然后利用液相色谱-电喷雾串联质谱(LC-ESI-MS/MS)方法鉴定蛋白,与Uniprot的细菌蛋白数据库进行比对从而鉴定褐飞虱成虫唾液中的细菌蛋白。【结果】在褐飞虱成虫唾液中鉴定到22种细菌的35种蛋白,这些蛋白主要参与能量代谢过程、蛋白的折叠和合成以及氨基酸代谢过程。这些细菌分布在变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)3个门。蛋白种类较多来源于变形菌门。【结论】鉴定到蛋白的这些细菌可能在褐飞虱的生活史中扮演重要角色。【Aim】To understand the bacteria species in saliva of adults of the brown planthopper,Nilaparvata lugens,by identifying the bacterial proteins in their saliva.【Methods】Saliva of N.lugens adults was collected by stretching two layers of Parafilm with sucrose diet.Protein solution was subjected to electrophoresis after concentration by ultrafiltration.Protein identification was conducted by liquid chromatography-electrospray ionisation-tandem mass spectrometry（LC-ESI-MS/MS） analysis,and the spectra were searched against the Uniprot database of bacterial protein to identify the bacterial proteins in saliva.【Results】 Thirty five proteins of twenty-two species of bacteria,mainly related to energy metabolism,protein folding and synthesis and amino acid metabolism,were identified in saliva of N.lugens adults.These bacteria belong to Proteobacteria,Actinobacteria and Firmicutes,and most are from Proteobacteria.【Conclusion】The endosymbiont bacteria in N.lugens,whose proteins were identified,may play an important role in the life history of this insect.

关 键 词：褐飞虱 唾液 细菌蛋白 共生菌 LC-ESI-MS/MS 

分 类 号：Q966[生物学—昆虫学]