杀虫蛋白Vip3Aa11对亚洲玉米螟及其寄生性天敌腰带长体茧蜂的影响  

Effects of Vip3Aa11 protein on Ostrinia furnacalis(Lepidoptera:Pyralidae) and its parasitoid Macrocentrus cingulum(Hymenoptera:Braconidae)

作　　者：郝杰[1,2] 王振营[2] 王勤英[1] 白树雄[2] 张天涛[2] 南宫自艳[1] 何康来[2] HAO Jie WANG Zhen-Ying WANG Qin-Ying BAI Shu-Xiong ZHANG Tian-Tao NANGONG Zi-Yan HE Kang-Lai(College of Plant Proteetion, Hebei Agrieuhural University, Baoding, Hebei 071000, China State Key Laboratory for Biology of Plant Diseases and Inseet Pests, Institute of Plant Protection, Chinese Academy of Agrieultural Sciences, Beijing 100193, China） Abstract： The aim of this study is to clarify the effects of Vip3Aall toxin on the Asian corn borer, Ostrinia furnaealis, and its parasitoid Maeroeentrus cingulum. The indireet effects of)

机构地区：[1]河北农业大学植物保护学院,河北保定071000 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2017年第7期817-824,共8页Acta Entomologica Sinica

基　　金：国家转基因生物新品种培育科技重大专项(2016ZX08003)

摘　　要：【目的】本文的研究目的是明确杀虫蛋白Vip3Aa11对亚洲玉米螟Ostrinia furnacalis及其寄生性天敌腰带长体茧蜂Macrocentrus cingulum的影响。【方法】首先利用生物测定的方法用含100μg/g Vip3Aa11蛋白的人工饲料饲喂亚洲玉米螟初孵幼虫,7 d后观察记录亚洲玉米螟的死亡情况和体重。然后用含100μg/g Vip3Aa11蛋白的人工饲料饲喂被腰带长体茧蜂寄生的亚洲玉米螟初孵幼虫,以含6μg/g印楝素的人工饲料作为阳性对照,10 d后记录亚洲玉米螟幼虫的死亡和被寄生情况,并在茧蜂化蛹结茧及羽化后分别记录茧重以及单个寄主的出蜂量,以此评价Vip3Aa11蛋白对腰带长体茧蜂的间接影响。再者,用含100μg/g Vip3Aa11蛋白的20%蜂蜜水饲喂腰带长体茧蜂成虫,以含100μg/g印楝素的20%蜂蜜水作为阳性对照,观察记录腰带长体茧蜂成蜂的死亡情况;不同处理的腰带长体茧蜂寄生亚洲玉米螟10 d后记录亚洲玉米螟的死亡和被寄生情况,并在子代茧蜂化蛹结茧及羽化后记录茧重及单个寄主的出蜂量,以此评价Vip3Aa11蛋白对腰带长体茧蜂的直接影响。【结果】生物测定结果显示,100μg/g的Vip3Aa11蛋白处理7 d后亚洲玉米螟幼虫的平均死亡率为50.7%,平均体重抑制率为77.1%。间接影响试验结果显示,被腰带长体茧蜂寄生后的亚洲玉米螟幼虫取食含有Vip3Aa11蛋白的人工饲料后死亡率明显升高,腰带长体茧蜂茧重和单头出蜂量大幅度下降,而寄生率及羽化后的成虫寿命没有受到不利影响。直接影响试验结果显示,腰带长体茧蜂取食含有100μg/g Vip3Aa11蛋白的蜂蜜水对腰带长体茧蜂成虫寿命和寄生率、亚洲玉米螟幼虫死亡率以及腰带长体茧蜂子代的茧重、单头出蜂量和成虫寿命均没有产生不利影响。【结论】本研究利用生测体系从直接和间接两方面评价了Vip3Aa11蛋白对腰带长体茧蜂的影响,结果表明腰带长体茧蜂对高于B【Aim】The aim of this study is to clarify the effects of Vip3Aa11 toxin on the Asian corn borer,Ostrinia furnacalis,and its parasitoid Macrocentrus cingulum.【Methods】The indirect effects ofVip3Aa11 protein on M.cingulum were assessed by the bioassay with the artificial diet containing 6 μg/g azadirachtin used as the positive control.The mortality and parasitism of O.furnacalis larvae parasitized by M.cingulum were recorded at 10 d after feeding with a diet containing 100 μg/g Vip3Aa11 protein,and the cocoon weight of M.cingulum and the emergence number per O.furnacalis larva after pupation and emergence of M.cingulum were investigated.The direct effects of Vip3Aa11 protein on M.cingulum were assessed by the bioassay with 20% honey solution containing 100 μg/g azadirachtin used as the positive control.The mortality of M.cingulum adults daily after feeding with 20% honey solution containing 100 μg/g Vip3Aa11 protein,and the mortality and parasitism rate of O.furnacalis larvae at 10 d after being parasitized by M.cingulum adults from different treatments were recorded.The cocoon weight of M.cingulum and emergence number per O.furnacalis larva of next generation of M.cingulum were recorded after pupation and emergence.【Results】The bioassay results showed that the average mortality and average inhibition rate of body weight of O.furnacalis larvae were50.7% and 77.1%,respectively,at 7 d after exposure to 100 μg/g Vip3Aa11 protein.In the bioassay of indirect effects,the mortality of O.furnacalis larvae significantly increased when they were fed with the artificial diet containing Vip3Aa11 protein after parasitized by M.cingulum,and the cocoon weight and the number of M.cingulum progenies produced per host decreased;however,the toxin had no effect on the parasitism rate and longevity of M.cingulum adults.In the bioassay of direct effects,the longevity and parasitism rate of M.cingulum adults,the mortality of O.furnacalis larvae,and the cocoon weight,the number of progenies produced per host and the adult

关 键 词：腰带长体茧蜂 亚洲玉米螟 杀虫蛋白 Vip3Aa11 生物测定 寄生 

分 类 号：Q968[生物学—昆虫学]