气相色谱串联质谱法测定卷烟主流烟气中的B[a]P  

Determination of Benzo[a]pyrene in Mainstream Cigarette Smoke by Gas Chromatography-tandem Mass Spectrometry

作　　者：蒋惠智 姬厚伟 黄淼 杨超 惠非琼 杨敬国 刘剑 JIANG Hui-zhi JI Hou-wei HUANG Miao YANG Chao HUI Fei-qiong YANG Jing-guo LIU Jian(Technology Center of China Tobacco Guizhou Industrial Co. , Ltd. , Guizhou Guiyang 550009, Chin)

机构地区：[1]贵州中烟工业有限责任公司技术中心,贵州贵阳550009

出　　处：《广州化工》2017年第15期122-124,165,共4页GuangZhou Chemical Industry

基　　金：贵州中烟工业有限责任公司科技项目"贵州中烟配方模块烟气中重要致香成分分析方法的研究"(201019)

摘　　要：建立了超声萃取-气相色谱-串联质谱法(GC-MS/MS)同时测定卷烟主流烟气中苯并[a]芘的分析方法。捕集了卷烟主流烟气总粒相物的剑桥滤片经环己烷超声萃取,在选择反应监测(MRM)模式下进行GC-MS/MS分析,内标法定量。结果表明:苯并[a]芘线性良好,r2为0.9996,方法的检出限(LOD)和定量限(LOQ)分别为0.4 ng/支和0.8 ng/支,加标回收率为98.2%~99.3%,相对标准偏差为1.7%~6.6%。该方法操作简便、灵敏,适合用于卷烟主流烟气"中苯并[a]芘释放量的测定。A gas chromatography-tandem mass spectrometry （GC-MS/MS） method was successfully developed and validated for determination of benzo [ a ] pyrene in main cigarette smoke. The total particulate matters （TPMs） captured by Cambridge filter pads was extracted by ultrasound extraction with cyclohexane and analyzed by GC-MS/MS under multi- reaction monitoring （MRM）mode, and quantified by internal standard method. The results showed that the calibration curves showed good linearity for benzo[ a ] pyrene and the correlation coefficients was 0. 9996. The limits of detection and limits of quantification for 8 alkaloids were 0. 4 ng/cig and 0. 8 ng/cig, respectively. Recoveries were in the range of 98.2% to 99.3% and the relative standard deviations （RSDs） were 1.7% to 6.6%. The proposed method was simple, sensitive and suitable for the determination of benzo [ a ] pyrene in cigarette mainstream smoke.

关 键 词：卷烟 主流烟气 超声萃取 GC-MS/MS 苯并[A]芘 

分 类 号：TS411[农业科学—烟草工业]