血根碱亚慢性毒性试验组织病理学观察  

Histopathological observation on sub-chronic toxicity of sanguinarine in mice

作　　者：伍勇[1,2] 肖倩文 邢东梅[2] 宋璐琳 张孜婕 孙卓[2] 孙志良[2,3] WU Yong XIAO Qianwen XING Dongmei SONG Lulin ZHANG Zijie SUN Zhuo SUN Zhiliang(College of Orient Science and Technology, Hunan Agricultural University, Changsha ttunan 410128, China College of Veterinary Medicine, ttunan Agricultural University, Changsha ttunan 410128, China Hunan Engineering Research Center of Veterinary Drug, Changsha Hunan 410128, China)

机构地区：[1]湖南农业大学东方科技学院,湖南长沙410128 [2]湖南农业大学动物医学院,湖南长沙410128 [3]湖南省兽药工程技术研究中心,湖南长沙410128

出　　处：《中兽医医药杂志》2017年第4期44-46,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然科学基金项目(31402255);湖南农业大学东方科技学院青年基金项目(14QNZ13)

摘　　要：观察血根碱亚慢性毒性试验对小鼠脏器的组织病理学变化,为将血根碱应用于兽医临床提供实验依据。将血根碱分别按高剂量[21 mg/(kg·d)]、低剂量[2.1 mg/(kg·d)]以及蒸馏水给小鼠连续灌胃30 d后,计算脏器指数,并观察各脏器的组织病理学变化。结果显示,与空白组相比,21 mg/(kg·d)剂量组小鼠脾脏与肾脏出现组织病理学变化,2.1 mg/(kg·d)剂量组各脏器未见病理变化,且小鼠脾脏指数降低,其他脏器指数无变化。本研究对血根碱的亚慢性毒性进行了初步评价,为血根碱的临床应用提供理论基础。To observe the histopathologi cal change of sub-chronic toxicity of sanguinarine in mice, two treatment groups and control group were divided up. Mice in high-dose and low-dose groups were respectively administrated sanguinarine at 21 mg/（kg·d） and 2.1 mg/（kg·d） orally for 30 consecutive days. The organ indexes were calculated and histopathological examination was carried by paraffin section technique and H.E straining method. The results showed that two treatment groups had no significant difference in histopathological change except spleen and kidney of high-dose group compared with the blank group. And there was no significant difference in organ indexes compared with the blank group except spleen index. The sub-chronic toxicity evaluation of sanguinarine laid a theoretical foundation for its clinical application.

关 键 词：血根碱 小鼠 脏器指数 组织病理学 

分 类 号：S853.7[农业科学—临床兽医学]