小檗碱对大肠杆菌基因转录抑制作用的实验研究  

Experimental Study on Inhibition Effect of Berberine in Escherichia coli Gene Transcription

作　　者：李慧玉[1] 王玉刚[1] 袁梽漪[2] 雷帆[3] 王欣佩[1] 卢希[1] 邢东明[1] 李俊[4] 杜力军[1] Li Huiyu Wang Yugang Yuan Zhiyi Lei Fan Wang Xinpei Lu Xi Xing Dongming Li Jun Du Lijun(Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China College of Pharmacy, Chongqing Medical University, Chongqing 400016, China School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China State Key Laboratory of Innovative Drugs and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang 330006, China)

机构地区：[1]清华大学生命科学学院药物药理实验室,北京100084 [2]重庆医科大学药学院,重庆400016 [3]清华大学药学院,北京100084 [4]江西中医药大学创新药物与高效节能降耗制药设备国家重点实验室,南昌330006

出　　处：《世界科学技术-中医药现代化》2017年第4期569-577,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委重大研究计划培育项目(90713043):基于BBR神经元保护作用探讨其PI3-K/AKT作用的始动位点及其信号转导;负责人:杜力军;国家自然科学基金委面上项目(81374006):基于BBR作为DNA插入子探讨其对正常与缺血再灌神经元基因转录作用差异的机制;负责人:邢东明

摘　　要：目的:对小劈碱(BBR)对大肠杆菌的抑制作用的分子靶点进行探讨。方法:鉴于BBR对DNA结合的偏好性,本文从基因转录表达水平对BBR的大肠杆菌生长抑制作用靶点进行实验研究。结果:BBR对于大肠杆菌基因上游调控元件UP element具有较强的亲和力,而在此元件地转录起始区含有TATA碱基序列。进一步对启动子上游含有UP element调控元件的基因sulA、recA、16S和启动子上游不含UP element调控元件的基因lpxC、secG、mutT的mRNA表达比较表明,BBR抑制sulA、recA、16S的表达,对lpxC、secG、mutT无明显作用,提示TATA序列是BBR的作用靶点。结论:本结果对从基因转录水平探讨BBR抑菌作用提供了新思路。There have been many reports on berberine （BBR） effect of the inhibition on gut bacteria, but more from the protein level. In view of the preference of BBR for DNA binding, we here investigated the expression of BBR from the transcriptional expression level of the gene. The resuhs showed that BBR had a higher affinity for UP element of Escherichia coli （E. coli） gene, and the transcription initiation region of this element contained TATA base sequence. The expression of genes sulA, recA and 16S which contain the genes of the UP element regulatory elements in the upstream of the promoter could be suppressed by BBR, and the expression of lpxC, secG and mutt which did not contain the genes of the UP element regulatory elements in the upstream of the promoter could not be inhibited by BBR. It is shown that the TATA sequence is the target of BBR. This result provides a new perspective for exploring the effect of BBR＇s inhibition of microbiota from gene transcription.

关 键 词：小檗碱 大肠杆菌 基因转录 TATA BOX 

分 类 号：R285[医药卫生—中药学]