鞘内注射MK801和美金刚对脊髓神经元凋亡的影响  

Effects of intrathecal injection of MK801 and Memantine on apoptosis of neurons in spinal cord

作　　者：陈洋洋[1] 石依倩 王云[1] CHEN Yangyang SHI Yiqian WANG Yun(Brain Res Inst, Fudan Univ, Shanghai 200032, Chin)

机构地区：[1]复旦大学脑科学研究院,上海200032

出　　处：《扬州大学学报（农业与生命科学版）》2017年第2期21-27,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：上海市科研计划重大基础研究项目(13DJ1400302)

摘　　要：通过小鼠鞘内注射地佐环平(MK801,3、10nmol·L-1)和美金刚(10、30nmol·L-1),运用免疫组织化学染色的方法,探讨这2种药物是否对脊髓神经元产生促进凋亡的作用。结果表明:小鼠鞘内注射MK801和美金刚对脊髓背角神经元(主要为感觉神经元)、腹角神经元(主要为运动神经元)及其他细胞均无促凋亡作用,且对腰段脊髓横切面上的细胞数量无显著影响。该试验为研究NMDA受体在脊髓层面作为用药靶点的安全用药提供了基础和前提。The experimental mice were given MK801（3,10nmol·L^-1）and Memantine（10,30nmol·L^-1）by intrathecal administration to explore whether these drugs would induce the apoptosis of spinal cord neurons via immunostaining method.The results showed that there were no positive TUNEL signals in spinal dorsal horn（mainly sensory neurons）,spinal ventral horn（mainly motor neurons）neurons and other kind of neurons.As for the number of cells in the transverse section of lumbar spinal cord,there were no significant differences across all of the six groups.This study provided the basis and premise for studying the specific target of NMDA receptor in spinal cord in clinical safe pharmacy treatment.

关 键 词：NMDA受体 NMDA受体拮抗剂 MK801 美金刚 凋亡 

分 类 号：Q423[生物学—神经生物学]